Absorbance modulated fluorescence detection methods and sensors

Details Absorbance modulated fluorescence detection methods and sensors Absorbance modulated fluorescence detection methods and sensors

1989-02-02

1992-09-01

Kepplinger, Esther L.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

436518, 436528, 436536, 436546, 436172, 436800, 436805, 436 56, 436164, 436165, 435808, G01N 3353, G01N 33536, G01N 2100

Patent

active

051438534

ABSTRACT:
A variety of methods and apparatus for the detection of an analyte of interest in a fluid sample is provided which relies upon the interaction of a fluorophore and a chromophoric light absorbing compound for qualitative and quantitative results. The methods preferably employ fiber optic sensors in combination with fluorophores and/or proto-absorber substances in mobile and immobilized modes of use. The methods and apparatus rely upon the ability of the light absorbing compositions to absorb energy which is transferred either radiatively or non-radiatively by the fluorophore when in an excited state.

REFERENCES:
patent: 4174384 (1979-11-01), Ullman et al.
patent: 4495293 (1985-01-01), Shaffar
patent: 4929561 (1990-05-01), Hirshfeld

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