Chemistry: analytical and immunological testing – Peptide – protein or amino acid
Patent
1997-03-20
1998-06-09
Snay, Jeffrey
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
436164, 436178, G01N 3368
Patent
active
057632820
ABSTRACT:
A rapid, practical assay for measuring the concentration of cornified envelopes based on measuring the absorbance, which utilizes the addition of bicinchoninic acid to insoluble cornified envelopes preparation, after the cornified envelopes have been separated from soluble proteins.
REFERENCES:
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patent: 5506144 (1996-04-01), Sundrehagen
patent: 5654171 (1997-08-01), Suginaka et al.
Monroe et al., "Quantitation of Cross-Linked Protein: An Alternative to Counting Cornified Envelopes as an Index of Keratinocyte Differentiation", Analytical Biochemistry, 199, pp. 25-28 (1991).
Lowry et al., "Protein Measurement With The Folin Phenol Reagent", Washington University School of Medicine, St. Louis, Missouri, May 28, 1951, pp. 265-275.
Sun et al., "Differentiation of the Epidermal Keratinocyte in Cell Culture: Formation of the Cornified Envelope", Cell, vol. 9, pp. 511-521, Dec. 1975(Part 1).
Stich, "Determination of Protein Covalently Bound to Agarose Supports Using Bicinchoninic Acid", Analytical Biochemistry, 191, pp. 343-346 (1990).
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Hill et al., "Protein Determination Using Bicinchoninic Acid in the Presence of Sulfhydryl Reagents", Analytical Biochemistry, 170, pp. 203-208 (1988).
Pierce Chemical Company, Product Brochure Instructions, "BCA Protein Assay Reagent Kit", USA, Jun. 1996.
Pierce Chemical Company, Product Brochure Instructions, "BCA Protein Assay Reagent", USA, Jul. 1995.
Chesebrough-Pond's USA Co. Division of Conopco, Inc.
Mitelman Rimma
Snay Jeffrey
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