Absolute quantitation of protein contents based on...

Details Absolute quantitation of protein contents based on... Absolute quantitation of protein contents based on...

2011-03-22

2011-03-22

Gakh, Yelena G (Department: 1777)

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

C702S019000

Reexamination Certificate

active

07910372

ABSTRACT:
The present inventor has established protein abundance index (PAI, π) to determine the protein contents in a protein mixture solution using nanoLC-MSMS data. Digested peptides were analyzed by nanoLC-MS/MS and the obtained results were applied to a Mascot protein identification algorism based on tandem mass spectra. For absolute quantitation, PAI was converted to exponentially modified PAI (EMPAI, m π), which is proportional to protein contents in the protein mixture. EMPAI was successfully applied to comprehensive protein expression analysis and performed a comparison study between gene and protein expression in an HCT116 human cancer cells. Accordingly, the present invention provides a method and a computer program for quantifying the protein contents based on the protein abundance index.

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