AAV2 Rep protein fusions

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S320100, C536S023100, C530S350000

Reexamination Certificate

active

07122348

ABSTRACT:
This invention pertains to methods for promoting stable and site-specific integration of rep deleted recombinant adeno-associated virus vectors which result in less variable transgene expression and increased safety. These vectors are useful for delivery of a functional gene product to the desired intracellular location.

REFERENCES:
patent: 6627617 (2003-09-01), Samulski et al.
Antoni et al. 1991. J. Virology 65(1): 396-404.
Dilber, M.S., et al., “Intercellular delivery of thymidine kinase prodrug activating enzyme by the herpes simplex virus protein, VP22,”Gene Therapy6:12-21, 1999.
Elliott, Gillian, and Peter O'Hare, “Intercellular Trafficking and Protein Delivery by a Herpesvirus Structural Protein,”Cell88:223-233, Jan. 24, 1997.
Elliott, Gillian, and Peter O'Hare, Intercellular Trafficking of VP22-GFP fusion proteins,Gene Therapy6:149-151, 1999.
Elliott, Gillian, and Peter O'Hare, “Herpes Simplex Virus Type 1 Tegument Protein VP22 Induces the Stabilization and Hyperacetylation of Microtubles,”Journal of Virology72(8):6448-6455, Aug. 1998.
Elliott, Gillian, and Peter O'Hare, “Cytoplasm-to-Nucleus Translocation of a Herpesvirus Tegument Protein during Cell Divison,”Journal of Viroloy74(5):2131-2141, Mar. 2000.
Fang, B., et al., “Intercellular trafficking of VP22-GFP fusion proteins is not observed in cultured mammalian Cells,”Gene Therapu5:1:1420-1424, 1998.
Kotin, Robert M., et al., “Site-specific integration by adeno-associated virus,”Proc. Natl. Acad. Sci. USA87:2211-2215, Mar. 1990.
Nagahara, Hikaru, et al., “Transduction of full-length TAT fusion proteins onto mammalian cells: TAT-p27Kip1induces cell migration,”Nature Medicine4(12):1449-1452, Dec. 1998.
Phelan, Anne, et al., “Intercellular delivery of functional p53 by the herpesvirus protein VP22,”Nature Biotechnology16:440-443, May 1998.
Podsakoff, Greg, et al., “Efficient Gene Transfer into Nondividing Cells by Adeno-Associated Virus-Based Vectors,”Journal of Virology68(9):5656-5666, 1994.
Rinaudo, Daniela, et al., “Conditional Site-Specific Integration into Human Chromosone 19 by Using a Ligand-Dependent Chimeric Adeno-Associated Virus/Rep Protein,”Journal of Virology74(1):281-294, Jan. 2000.
Samulski, R.J., et al., “Targeted integration of adeno-associated virus (AAV) into human chromosone 19,”The EMBO Journal10(12):3941-3950, 1991.
Schwarze, Steven R., et al., “In Vivo Protein Transduction: Delivery of a Biologically Active Protein into the Mouse,”Science285:1569-1572, Sep. 3, 1999.
Schwarze, Steven R., and Steven F. Dowdy, “In vivoprotein transduction: intracellular delivery of biologically active proteins, compounds and DNA,”Trends in Pharmacol. Sci.21:45-48, Feb. 2000.
Surosky, Richard T., et al., “Adeno-Associated Virus Rep Proteins Target DNA Sequences to a Unique Locus in the Human Genome,”Journal of Virology71(10):7951-7959, Oct. 1997.
Lacy, Elizabeth, et al., “A Foreign β-Globin Gene in Transgenic Mice: Integration at Abnormal Chromosomal Positions and Expression in Inappropriate Tissues,”Cell34:343-358, Sep. 1993.
Rivadeneira, Emilia D., et al., “Sites of recombinant adeno-associated virus integration,”International Journal of Oncology12:805-810, 1998.
Fisher-Adams, Grace, et al., “Integration of Adeno-Associated Virus Vectors in CD34+ Human Hematopoietic Progenitor Cells After Transduction,”Blood88(2): 492-504, Jul. 15, 1996.
Guis, David R., et al., “Transduced p16INK4aPeptides Inhibit Hypophosphorylation of the Retinoblastoma Protein and Cell Cycle Progession Prior to Activation of Cdk2 Complexes in Late G11,”Cancer Research59:2577-2580, Jun. 1, 1999.
Aints, Alar, et al., “Intercellular Spread of GFP-VP22,”The Journal of Gene Medicine1:275-279, 1999.
Chatterjee, Saswati, et al., “Dual-Target Inhibition of HIV-1 in Vitro by Means of an Adeno-Associated Virus Antisense Vector,”Science258:1485-1488, Nov. 27, 1992.
Derer, Wolfgang, et al., “Direct protein transfer to terminally differentiated muscle cells,”J. Mol. Med.77:609-613, 1999.

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