Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1983-04-26
1986-05-13
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351723, 435255, 435317, 935 28, 935 37, 935 69, 536 27, C12P 2100, C12N 1500, C12N 116, C12N 100
Patent
active
045886849
ABSTRACT:
Novel DNA constructs are provided for efficient expression of polypeptides by yeasts. The constructs employ yeast a-factor secretion leader and processing signals joined to a DNA sequence encoding a polypeptide of interest in reading frame with the a-factor signals. The constructions provide for the expression, secretion and maturation of the desired polypeptide. A strategy is provided for the isolation of the a-factor secretion leader and processing signals and the joining, by means of a relatively short adaptor, molecules of the DNA sequence encoding the polypeptide to the processing signals in proper reading frame.
The bacterial cell strain E. coli HB101 (pAB163) was deposited at the A.T.C.C. on Apr. 20, 1983 and given Accession No. 39342.
REFERENCES:
patent: 4338397 (1982-07-01), Gilbert et al.
Kurjan et al., Cell, V. 30, pp. 933-943, Oct. 1982, "Structure of a Yeast Pheromone Gene (MF.alpha.): A Putative a-Factor Precursor Contains Four Tandem Copies of Mature a-Factor".
Thorner, pp. 160-165, The Molecular Biology of the Yeast Saccharomyces Life Cycle and Inheritance, Cold Spring Harbor 1981 Article: "Yeast Pheromones and Mating".
Schekman et al., pp. 361-387, The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance, 1982, Cold Spring Harbor Press Article: "The Secretary Process and Yeast Cell Surface Assembly".
Astell et al., Cell, V. 27, pp. 15-23, "The Sequence of the DNAs Coding for the Mating-Type Loci of Saccaromyces cerevisiae".
Grantham et al., Nucleic Acids Research, May 10, 1980, vol. 8, No. 9, "Codon Frequencies in 119 Individual Genes Confirm Consistent Choices of Degenerate Bases According to Genome Type".
Nasmyth et al., Cold Spring Harbor Symposium on Quantitative Biology, 1981, vol. 45(2), pp. 961-981.
Beggs, Nature, vol. 275, Sep. 14, 1978, pp. 104-109, "Transformation of Yeast by a Replicating Hybrid Plasmid".
Davis et al., Nature, vol. 283, Jan. 1980, pp. 433-438, "The Mechanism of Protein Secretion Across Membranes".
Chiron Corporation
Rowland Bertram I.
Teskin Robin Lyn
Wiseman Thomas G.
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