7SK RNA regulated transcription

Details 7SK RNA regulated transcription 7SK RNA regulated transcription

2006-08-08

2006-08-08

Schultz, James (Department: 1635)

Chemistry: analytical and immunological testing

Process or composition for determination of physical state...

Corrosion resistance or power

C536S023100, C536S024300, C536S024500, C435S006120, C435S325000, C435S375000, C514S04400A

Reexamination Certificate

active

07087433

ABSTRACT:
A method for altering transcription in a cell comprising an amount of active CDK9/cyclin, comprises the steps of: (a) introducing in the cell an agent which modulates the amount of active CDK9/cyclin in the cell, and thereby alters transcription in the cell, wherein the agent comprises an RNA selected from the group consisting of an RNA aptamer that specifically binds CDK9/cyclin, a CDK9/cyclin-binding domain of 7SK RNA, a 7SK RNA-binding antisense 7SK RNA domain, and a 7SK RNA-specific RNAi, and (b) detecting a resultant altering of transcription in the cell.Methods for screening for an agent which modulates 7SK RNA-CDK9/cyclin binding generally comprise the steps of (a) incubating a mixture of 7SK RNA, CDK9/cyclin and a candidate agent under conditions wherein but for the presence of the agent, the 7SK RNA and CDK9/cyclin engage in a reference binding; and (b) detecting an agent-biased binding of the 7SK RNA to the CDK9/cyclin.

REFERENCES:
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Luo et al., C-Myc Deregulation During Transformation Induction: Involvement of 7SK RNA. 1997. Journal of Cellular Biochemistry, vol. 64, pp. 313-327.
Nguyen et al., 7SK small nuclear RNA binds and inhibits the activity of CDK9/cyclin T complexes, 2001, Nature, vol. 414, pp. 322-325.
Bieniasz et al., Recruitment of cyclin T1/P-TEFb to an HIV type 1 long terminal repeat promoter proximal RNA target is both necessary and sufficient for full activation of transcription, 1999, Proc. Natl. Acad. Sci., vol. 96, pp. 7791-7796.
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