7-Phenylacetic acid-4-alkyl-coumarinyl amides useful in fluorome

Details 7-Phenylacetic acid-4-alkyl-coumarinyl amides useful in fluorome 7-Phenylacetic acid-4-alkyl-coumarinyl amides useful in fluorome

1987-04-30

1988-10-11

Fan, Jane T.

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

435 18, C07D31116

Patent

active

047772697

ABSTRACT:
7-Phenylacetic acid-4-alkyl-coumarinyl amides having the formula ##STR1## wherein R is alkyl having 1 to 4, in particular 1 to 2 carbon atoms, optionally substituted by one or more halogen atoms, in particular fluorine atoms, especially methyl or trifluoromethyl, are suitable substances for the fluorometric determination of the activity of hydrolases, in particular of penicillin G-acylase, since by the enzymatic reaction they provide cleavage products which in regard to their fluorescence properties differ drastically from those of the substrate. They can be used in processes for the fluorometric determination of the activity of hydrolases, in particular of penicillin G-acylase.

REFERENCES:
patent: 2881186 (1959-04-01), Hausermann
patent: 3352885 (1967-11-01), Schelihammer et al.
patent: 4388233 (1983-06-01), Bissell et al.
patent: 4409140 (1983-10-01), Smith et al.

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