Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Reexamination Certificate
2002-03-28
2011-11-29
Ha, Julie (Department: 1654)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
C536S054000, C530S350000, C530S395000
Reexamination Certificate
active
08067196
ABSTRACT:
Disclosed are methods of 6-O-sulfating glucosaminyl N-acetylglucosamine residues (GlcNAc) in a polysaccharide preparation and methods of converting anticoagulant-inactive heparan sulfate to anticoagulant-active heparan sulfate and substantially pure polysaccharide preparations may by such methods. Also disclosed is a mutant CHO cell which hyper-produces anticoagulant-active heparan sulfate. Methods for elucidating the sequence of activity of enzymes in a biosynthetic pathway are provided.
REFERENCES:
patent: 5834282 (1998-11-01), Habuchi et al.
patent: 5955347 (1999-09-01), Lowe
patent: 6365365 (2002-04-01), Bistrup et al.
Merriam-Webster Online Dictionary-definition of variant. Accessed May 14, 2008.
Habuchi H, Tanaka M, Habuchi O, Yoshida K, Suzuki H, Ban K, Kimata K, “The Occurrence of Three Isoforms of Heparan Sulfate 6-O-sulfotransferase Havign Different Specificities for Hexuronic Acid Adjacent to the Targeted N-Sulfoglucosamine”, The Journal of Biological Chemistry, 2000, 275(4): 2859-2868.
Definition of derived from www.dictionary.com, pp. 1-5. Accessed Nov. 19, 2008.
Do A-T, Smeds E, Spillman D, Kusche-Gullberg M, “Overexpression of Heparan Sulfate 6-O-Sulfotransferases in Human Embryonic Kidney 293 Cells Results in Increased N-Acetylglucosaminyl 6-O-sulfation,” The Journal of Biological Chemistry, 2006, 281(9): 5348-5356.
Rosenbeerg RD, Shworak NW, Liu J, Schwartz JJ, Zhang L, “Perspectives Series: Cell Adhesion in Vascular Biology,” Journal of Clinical Investigation, 1997, 99(9): 2062-2070.
Lansdon EB, Fisher AJ, Segel IH, “Human 3′-phosphoadenosine 5′-Phosphosulfate Synthetase (Isoform 1, Brain): Kinetic Properties of the Adenosine Triphosphate Sulfurylase and Adenosine 5′-Phosphosulfate Kinase Domains,” Biochemistry, 2004, 43: 4356-5365.
Perrimon N and Bernfield M, “Specificities of heparan sulphate proteoglycans in developmental processes,” Nature, Apr. 13, 2000, 404: 725-728.
Cook BN, Bhakta, Biegel T, Bowman KG, Armstron JI, Hemmerich S, Bertozzi CR, “Differential Carbohydrate Recognition of Two GIcNAc-6-sulfotransferase with Possible Roles in L-Selectin Ligand Biosynthesis,” JACS, 2000, 122: 8612-8622.
Chatterton Jon E. et al, Expression cloning of LDLB, a gene essential for normal Golgi function and assembly of the IdICp complex, Proc. Natl. Acad. Sci, USA, vol. 96, pp. 916-920, Feb. 1999, Cell Biology.
Habuchi, Hiroko et al., Molecular Characterization and Expression of Heparan-sulfate 6-Sulfotransferase, The Journal of Biological Chemistry, vol. 273, No. 15, Issue of Apr. 10, pp. 9208-9213, 1998, The American Society for Biochemistry and Molecular Biology, Inc.
Hwang Sherry Lih-Hwa, et al, Expression of Genes Introduced Into Cells by etovirallnfection Is More Efficient than that of Genes Introduced Into Cells by DNA Transfection, Journal of Virology, May 1984, pp. 417-424, vol. 50, No. 2 1984, American Society for Microbiology.
Kitamura Toshio et al., Efficient screening of retroviral cDNA expression libraries, Proc. Natl. Acad, Sci, USA, vol. 92, pp. 9146-9150, Sep. 1995, Biochemistry.
Kjellen Lena, et al. Proteoglycans: Structures and Interactions, Annu. Rev. Biochem. 1991, 60:443-75, Annual Reviews Inc.
Liu Jian et al., Heparan Sulfact d-Glucosaminyl 3-0-Sulfotransferase-3A Sulfates N-Unsubstituted Glucosamine Residues, The Journal of Biological Chemistry, vol. 274, No. 53, Issue of Dec. 31, pp. 38155-38162, 1999, The American Society for Biochemistry and Molecular Biology, Inc.
Merry Catherine L. R. et al., Highly Sensitive Sequencing of the Sulfated Domains of Heparan Sulfate, The Journal of Biological Chemistry, vol. 274. No. 26, Issue of Jun. 25, pp. 18455-18462, 1999, The American Society of Biochemistry and Molecular Biology, Inc.
Rayner John R. et al., A Simple and Efficient Procedure for Generating Stable Expression Libraries by cDNA Cloning in a Retroviral Vector, Molecular and Cellular Biology, vol. 14, No. 2, Feb. 1994 pp. 880-887, American Society for Microbiology.
Shworak Nicholas W. et al., Multiple Isoforms of Heparan Sulfate D-Glucosaminyl 3-0-Sulfotransferase, The Journal of Biological Chemistry, vol. 274. No. 5, Issue of Feb. 19, pp. 5170-5184, 1999.
Wong Kenny K. et al. Engineering a Cell-Free Murein Biosynthetic Pahway: Combinatorial Enzymology in Drug Discovery, J. Am. Chem. Soc. 1998, 120, 13527-13528, American Chemical Society.
Wong Bryan Y. et al., High-Efficiency Identification of Genes by Functional Analysis from a Retroviral cDNA Expression Library, Journal of Virology, Sep. 1994, pp. 5523-5531, vol. 68. No. 9, American Society for Microbiology.
Zannettino Andrew C.W. et al. A Powerful New Technique for Isolating Genes Encoding Cell Surface Antigens Using Retroviral Expression Cloning, 1996 The American Association of Immunologists 156:611-620.
Zhang Lijuan et al. The Retinoic Acid and cAMP-Dependent Up-regulation of 3-0-Sulfotransferase-1 Leads to a Dramatic Augmentation of Anticoagulantly Active Heparan Sulfate Biosynthesis in F9 Embryonal Carcinoma Cells, The Journal of Biological Chemistry, vol. 273, No. 43 Issue of Oct. 23, pp. 27998-26003, 1998 The American Society for Biochemistry and Molecular Biology, Inc.
Zhang Lijuan et al., The Effect of Precursor structures on the Action of Glucosaminyl 3-0-Sulfotransferase-1 and the Biosynthesis of Anticoagulant Heparan Sulfate, The Journal of Biological Chemistry, vol. 276, No. 31 Issue of Aug. 3, pp. 28806-28813, 2001 The American Society of Biochemistry and Molecular Biology, Inc.
Beeler David L.
Rosenberg Robert D.
Zhang Lijuan
Cohen Mark S.
Ha Julie
Massachusetts Institute of Technology
Pearl Cohen Zedek Latzer LLP
LandOfFree
6-O sulfated polysaccharides and methods of preparation thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with 6-O sulfated polysaccharides and methods of preparation thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and 6-O sulfated polysaccharides and methods of preparation thereof will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4282504