6-aryloxy and arylthiopurines

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Heterocyclic carbon compounds containing a hetero ring...

Reexamination Certificate

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C514S234500, C514S241000, C514S261100, C514S262100, C514S263370, C514S265100, C514S266400, C544S061000, C544S118000, C544S212000, C544S266000, C544S267000, C544S268000, C544S269000, C544S270000, C544S271000, C544S273000, C544S276000

Reexamination Certificate

active

06258809

ABSTRACT:

FIELD OF THE INVENTION
This invention relates to novel compounds and pharmaceutical compositions, and to methods of using same in the treatment of psychiatric disorders and neurological diseases including major depression, anxiety-related disorders, post-traumatic stress disorder, supranuclear palsy and feeding disorders.
BACKGROUND OF THE INVENTION
Corticotropin releasing factor (herein referred to as CRF), a 41 amino acid peptide, is the primary physiological regulator of proopiomelanocortin(POMC) -derived peptide secretion from the anterior pituitary gland [J. Rivier et al.,
Proc. Nat. Acad. Sci.
(
USA
) 80:4851 (1983); W. Vale et al.,
Science
213:1394 (1981)]. In addition to its endocrine role at the pituitary gland, immunohistochemical localization of CRF has demonstrated that the hormone has a broad extrahypothalamic distribution in the central nervous system and produces a wide spectrum of autonomic, electrophysiological and behavioral effects consistent with a neurotransmitter or neuromodulator role in brain [W. Vale et al.,
Rec. Prog. Horm. Res.
39:245 (1983); G. F. Koob,
Persp. Behav. Med.
2:39 (1985); E. B. De Souza et al.,
J. Neurosci.
5:3189 (1985)]. There is also evidence that CRF plays a significant role in integrating the response of the immune system to physiological, psychological, and immunological stressors [J. E. Blalock,
Physiological Review
69:1 (1989); J. E. Morley,
Life Sci.
41:527 (1987)].
Clinical data provide evidence that CRF has a role in psychiatric disorders and neurological diseases including depression, anxiety-related disorders and feeding disorders. A role for CRF has also been postulated in the etiology and pathophysiology of Alzheimer's disease, Parkinson's disease, Huntington's disease, progressive supranuclear palsy and amyotrophic lateral sclerosis as they relate to the dysfunction of CRF neurons in the central nervous system [for review see E. B. De Souza,
Hosp. Practice
23:59 (1988)].
In affective disorder, or major depression, the concentration of CRF is significantly increased in the cerebral spinal fluid (CSF) of drug-free individuals [C. B. Nemeroff et al.,
Science
226:1342 (1984); C. M. Banki et al.,
Am. J. Psychiatry
144:873 (1987); R. D. France et al.,
Biol. Psychiatry
28:86 (1988); M. Arato et al.,
Biol Psychiatry
25:355 (1989)]. Furthermore, the density of CRF receptors is significantly decreased in the frontal cortex of suicide victims, consistent with a hypersecretion of CRF [C. B. Nemeroff et al.,
Arch. Gen. Psychiatry
45:577 (1988)]. In addition, there is a blunted adrenocorticotropin (ACTH) response to CRF (i.v. administered) observed in depressed patients [P. W. Gold et al.,
Am J. Psychiatry
141:619 (1984); F. Holsboer et al.,
Psychoneuroendocrinology
9:147 (1984); P. W. Gold et al.,
New Eng. J. Med.
314:1129 (1986)]. Preclinical studies in rats and non-human primates provide additional support for the hypothesis that hypersecretion of CRF may be involved in the symptoms seen in human depression [R. M. Sapolsky,
Arch. Gen. Psychiatry
46:1047 (1989)]. There is preliminary evidence that tricyclic antidepressants can alter CRF levels and thus modulate the numbers of CRF receptors in brain [Grigoriadis et al.,
Neuropsychopharmacology
2:53 (1989)].
There has also been a role postulated for CRF in the etiology of anxiety-related disorders. CRF produces anxiogenic effects in animals and interactions between benzodiazepine
on-benzodiazepine anxiolytics and CRF have been demonstrated in a variety of behavioral anxiety models [D. R. Britton et al.,
Life Sci.
31:363 (1982); C. W. Berridge and A. J. Dunn Regul.
Peptides
16:83 (1986)]. Preliminary studies using the putative CRF receptor antagonist a-helical ovine CRF (9-41) in a variety of behavioral paradigms demonstrate that the antagonist produces “anxiolytic-like” effects that are qualitatively similar to the benzodiazepines [C. W. Berridge and A. J. Dunn
Horm. Behav.
21:393 (1987),
Brain Research Reviews
15:71 (1990)]. Neurochemical, endocrine and receptor binding studies have all demonstrated interactions between CRF and benzodiazepine anxiolytics providing further evidence for the involvement of CRF in these disorders. Chlordiazepoxide attenuates the “anxiogenic” effects of CRF in both the conflict test [K. T. Britton et al.,
Psychopharmacology
86:170 (1985); K. T. Britton et al.,
Psychopharmacology
94:306 (1988)] and in the acoustic startle test [N. R. Swerdlow et al.,
Psychopharmacology
88:147 (1986)] in rats. The benzodiazepine receptor antagonist (Ro15-1788), which was without behavioral activity alone in the operant conflict test, reversed the effects of CRF in a dose-dependent manner while the benzodiazepine inverse agonist (FG7142) enhanced the actions of CRF [K. T. Britton et al.,
Psychopharmacology
94:306 (1988)].
The mechanisms and sites of action through which the standard anxiolytics and antidepressants produce their therapeutic effects remain to be elucidated. It has been hypothesized however, that they are involved in the suppression of the CRF hypersecretion that is observed in these disorders. Of particular interest is that preliminary studies examining the effects of a CRF receptor antagonist (a-helical CRF
9-41
) in a variety of behavioral paradigms have demonstrated that the CRF antagonist produces “anxiolytic-like” effects qualitatively similar to the benzodiazepines [for review see G. F. Koob and K. T. Britton, In:
Corticotropin
-
Releasing Factor: Basic and Clinical Studies of a Neuropeptide,
E. B. De Souza and C. B. Nemeroff eds., CRC Press p221 (1990)].
DuPont Merck PCT application US 94/11050 describes corticotropin releasing factor antagonist compounds of the formula:
and their use to treat psychiatric disorders and neurological diseases. Included in the description are fused pyridines and pyrimidines of the formula:
where: Z is CR
2
or N; A is CR
30
or N; D is CR
28
or N; and R
3
can be aryloxy or arylthio.
Pfizer WO 95/33750 describes corticotropin releasing factor antagonist compounds useful in the treatment of CNS and stress disorders. The description includes compounds of the formulae:
where A is CR
7
or N; B is OCHR
1
R
2
or SCHR
1
R
2
; R
1
is substituted or unsubstituted alkyl; R
2
is substituted or unsubstituted alkyl, aryl or heteroaryl; R
3
is methyl, halo, cyano, methoxy, etc.; R
4
is H, substituted or unsubstituted alkyl, halo, amino, nitro, etc.; R
5
is substituted or unsubstituted aryl or heteroaryl; R
6
is H or substituted or unsubstituted alkyl; R
7
is H, methyl, halo, cyano, etc.; R
16
and R
17
taken together form an oxo (═O) group; and G is ═O, ═S, ═NH, ═NCH
3
, hydrogen, methyl, methoxy, etc.
Pfizer WO 95/34563 describes corticotropin releasing factor antagonist compounds, including compounds of the formula:
where A, B and the R groups have definitions similar to those in WO 95/33750.
Pfizer WO 95/33727 describes corticotropin releasing factor antagonist compounds of the formula:
where A is CH
2
and Z can be a heteroaryl moiety.
Ganguly et al., U.S. Pat. No. 4,076,711 describes triazolo[4,5-d]pyrimidines of the formula:
where X is halo, —NR
1
R or alkoxy, with R1 and R each being H or alkyl; Y is alkyl, cycloalkyl, hydroxycycloalkyl, phenyl, bicycloalkyl or phenylalkyl or bicycloalkylalkyl; and Q is H or Y. The patent states that the compounds are useful in the treatment of psoriasis.
Tanji et al., Chem. Pharm. Bull. 39(11)3037-3040(1991), describes triazolo[4,5-d]pyrimidines of the formula:
where halo is I, Br or Cl, Ph is phenyl and Me is methyl. No utility for the compounds is described.
Settimo et al., Il Farmaco, Ed. Sc., 35 (4), 308-323 (1980) describes 8-azaadenines (triazolo[4,5-d] pyrimidines) of the formula:
where R1 is H or benzyl and R2 is p-methylphenyl.
Biagi et al., Il Farmaco, 49 (3), 183-186 (1994), describes N(6)-subst

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