5'to 3' nucleic acid synthesis using 3'-photoremovable protectin

Details 5'to 3' nucleic acid synthesis using 3'-photoremovable protectin 5'to 3' nucleic acid synthesis using 3'-photoremovable protectin

1995-03-16

1999-06-01

Kunz, Gary L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2533, C07H 102, C07H 100

Patent

active

059089261

ABSTRACT:
The present invention relates, in general, to a method of synthesizing a nucleic acid, and, in particular, to a method of effecting 5' to 3' nucleic acid synthesis. The method can be used to prepare arrays of oligomers bound to a support via their 5' end. The invention also relates to a method of effecting mutation analysis using such arrays. The invention further relates to compounds and compositions suitable for use in such methods.

REFERENCES:
patent: 4415732 (1983-11-01), Caruthers et al.
patent: 5143854 (1992-09-01), Pirrung et al.
patent: 5242974 (1993-09-01), Holmes
patent: 5486633 (1996-01-01), Pirrung et al.
patent: 5489678 (1996-02-01), Fodor et al.
Pirrung et al, "Alcohols. Asymmetric Synthesis of Phosphotriesters of (-)-3',5'-Dimethoxybenzoin", J. Org. Chem. 59(14):3890-3897 (1994).
Pirrung and Bradley, "Comparison of Methods for Photochemical Phosphoramidite-Based DNA Synthesis", J. Org. Chem., vol. 60, 1995, pp. 6270-6276.
Pirrung and Bradley, "Dimethoxybenzion Carbonates: Photochemically-Removable Alcohol Protecting Groups Suitable for Phosphoramidite-Based DNA Synthesis", J. Org. Chem., vol. 60, 1995, pp. 1116-1117.
Fodor et al, "Multiplexed biochemical assays with biological chips", Nature 364:555-556 (1993).
Fodor et al, "Light-Directed, Spatially Addressable Parallel Chemical Synthesis", Science 251:767-773 (1991).
Horne et al, "Recognition of Mixed-Sequence Duplex DNA by Alternate-Strand Triple-Helix Formation", J. Am. Chem. Soc. 112:2435-2437 (1990).
Pirrung et al, "Photochemically-Removable Silyl Protecting Groups", J. Org. Chem. 58:6961-6963 (1993).
Lew et al, Photochemical Protodesilylation of 2-R.sub.3 Si-1, 3-dimethoxybenzenes. Direct Observation of .beta.-Silyl-Subsstituted Cyclohexadienyl Cations, J. Am. Chem. Soc. 115:11516-11520 (1993).
Givens et al, "Photochemistry of Phosphate Esters", Chem. Rev. 93(1):55-66 (1993).

Affiliated with

Also associated with

Rating

5'to 3' nucleic acid synthesis using 3'-photoremovable protectin does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with 5'to 3' nucleic acid synthesis using 3'-photoremovable protectin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and 5'to 3' nucleic acid synthesis using 3'-photoremovable protectin will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-955680