5' to 3' exonuclease mutations of thermostable DNA polymerases

Details 5' to 3' exonuclease mutations of thermostable DNA polymerases 5' to 3' exonuclease mutations of thermostable DNA polymerases

1995-06-02

1998-08-18

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C12N 912

Patent

active

057957622

ABSTRACT:
The present invention relates to thermostable DNA polymerases which exhibit a different level of 5' to 3' exonuclease activity than their respective native polymerases. Particular conserved amino acid domains in thermostable DNA polymerases are mutated or deleted to alter the 5' to 3' exonuclease activity of the polymerases. The present invention also relates to means for isolating and producing such altered polymerases.

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patent: 4889818 (1989-12-01), Gelfand et al.
patent: 5079352 (1992-01-01), Gelfand et al.
patent: 5108892 (1992-04-01), Burke et al.
patent: 5210015 (1993-05-01), Gelfand et al.
Bernad et al., 1989, "A Conserved 3'-5' Exonuclease Active Site in Prokaryotic and Eukaryotic DNA Polymerases" Cell 59:219-228.
Lawyer et al., 1989, "Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene From Thermus Aquaticus" J. Biol. Chem 264(11):6427-6437.
Leavitt and Ito, 1989, "T5 DNA Polymerase: Structural-Functional Relationships to Other DNA Polymerases" Proc. Natl. Acad. Sci. USA 86:4465-4469.

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