5-methylcytosine detection, compositions and methods therefor

Details 5-methylcytosine detection, compositions and methods therefor 5-methylcytosine detection, compositions and methods therefor

2011-08-16

2011-08-16

Riley, Jezia (Department: 1637)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S024300, C536S026600, C435S006120

Reexamination Certificate

active

07999091

ABSTRACT:
Compositions and methods for detecting 5-methylcytosine in a nucleic acid are disclosed. A 5-methylcytosine discriminator, which is a deoxyribonucleosidetriphosphate comprising a cytosine-pairing moiety such as a guanosine and a moiety which hinders hydrogen bonding between the cytosine-pairing moiety and a 5-methylcytosine is described. The discriminator is able to base pair with a cytosine but not a 5-methylcytosine. A 5-methylcytosine comprised by a target nucleotide can be detected in a reaction using a DNA polymerase and a primer hybridized immediately adjacent to the target nucleotide. In the reaction, pyrophosphate released upon incorporation of a dNTP complementary to a target nucleotide is detected. Lack of incorporation of the discriminator, but incorporation of a dGTP, can indicate that the target nucleotide is a 5-methylcytosine.

REFERENCES:
patent: 2007/0042365 (2007-02-01), Millar et al.
patent: WO 2004113564 (2004-12-01), None

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