5'[2'(3')-O-(2,4,6-trinitrophenyl) pyprimidine nucleoside]diphos

Details 5'[2'(3')-O-(2,4,6-trinitrophenyl) pyprimidine nucleoside]diphos 5'[2'(3')-O-(2,4,6-trinitrophenyl) pyprimidine nucleoside]diphos

1988-12-06

1992-04-28

Brown, Johnnie R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 27, 536 28, 536 186, 536 6, 536 552, 536123, 435 6, C07H 1910

Patent

active

051091267

ABSTRACT:
A method of assaying, detecting, monitoring, and influencing in vitro and in vivo activity of glycosyltransferase and sugar nucleotides which are analogs of the naturally-occurring sugar nucleotides for which the glycosyltransferases are specific. These sugar nucleotide analogs include those labeled with a fluorogenic moiety at the 2' or 3' position of the ribose or at another location (e.g., on a constituent phosphate or in the nucleotide backbone), such as the UDP-galactose analog, 2'(or 3')-O-(2,4,6-trinitrophenyl)-5'-uridine diphosphate galactose (TUG). A highly specific assay for soluble glycosyltransferase has been developed which utilizes the fluorogenic sugar-nucleotide analogs. These assays rely on changes in spectral properties resulting from specific binding events of the sugar nucleotide analog and the glycosyltransferase. Assays of this invention do not rely on the use of radio-isotopes and can be used to assess glycosyltransferase activity in living cells.

REFERENCES:
patent: 3687932 (1972-08-01), Nakamachi et al.
patent: 4472572 (1984-09-01), Shizuya
patent: 4704361 (1987-11-01), Miccoli et al.
Hiratsuka et al., Biochimica et Biophysica Acta, 320, 635-647 (1973).
Michelson et al., J. Chem. Soc., 3459-3463 (1956).
Jacobson et al., Biochemistry, 22, 4247-4257 (1983).
Berman, J. Biol. Chem., 261(35), 16494-16501 (1986).
B. D. Shur and S. Roth, "Cell Surface Glycosyltransferases", Biochim. Biophys. Acta, 415:473-512 (1975).
Schwyzer, M. and R. L. Hill, J. Biol. Chem. 252:2338-2345 (1977).
E. G. Moczydlowski and P. A. George Fortes, J. Biol. Chem., 256:2357-2366 (1981).
Cardullo, R. A. et al. Abstract: Annual Meeting of Soc. of Cell Biology, 1988.

Affiliated with

Also associated with

Rating

5'[2'(3')-O-(2,4,6-trinitrophenyl) pyprimidine nucleoside]diphos does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with 5'[2'(3')-O-(2,4,6-trinitrophenyl) pyprimidine nucleoside]diphos, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and 5'[2'(3')-O-(2,4,6-trinitrophenyl) pyprimidine nucleoside]diphos will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1250665