Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-08-05
1995-06-13
Spiegel, Carol A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 75, 435 792, 435 793, 435 794, 435 795, 435 28, 435968, 435975, G01N 33543, G01N 33535
Patent
active
054241944
ABSTRACT:
A chemiluminescent reaction, which may be utilized as an immunoassay, carried out by 2,3-dihydro-1,4-phthalazinedione or its derivative, a peroxidase, and an oxidant, is enhanced by adding a phenolic compound such as (4-cyanomethylthio)phenol thereto. Especially, it is preferred that (4-cyanomethylthio)phenol is added to a mixture of luminol, horseradish peroxidase, and hydrogen peroxide to provide a significantly enhanced and stabilized luminescent reaction. Involving this enhanced luminescent reaction to an immunoassay, the sensitivity of the assay may be increased.
REFERENCES:
patent: 4598044 (1986-07-01), Kricka et al.
Catalogue of Aldrich Chemical Company (1990) p. 890.
Thorpe et al, Methods in Enzymology, vol. 133, pp. 331-353 (1986).
"Clinical Chem." H. G. Thorpe et al; vol. 31, No. 8. p. 1335 (1985).
Koizumi Tatsuya
Kouge Katsushige
Otagiri Ken
Sugiura Masakazu
Green Lora M.
Sanyo Chemical Industries Ltd.
Spiegel Carol A.
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