4,7-dichlororhodamine dyes labeled polynucleotides

Details 4,7-dichlororhodamine dyes labeled polynucleotides 4,7-dichlororhodamine dyes labeled polynucleotides

2004-02-26

2009-06-23

Riley, Jezia (Department: 1637)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S026600, C435S006120

Reexamination Certificate

active

07550570

ABSTRACT:
A set of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structureswherein R1-R6are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or, when taken together, R1and R6is benzo, or, when taken together, R4and R5is benzo; R7-R10, R12-R16and R18may be hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group; R11and R17may be hydrogen, lower alkyl, lower alkene, lower alkyne, phenyl, aryl, linking group; Y1-Y4are hydrogen, lower alkyl, or cycloalkyl, or, when taken together, Y1and R2, Y2and R1Y3and R3, and/or Y4and R4is propano, ethano, or substituted forms thereof; and X1-X3taken separately are hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonate, hydroxymethyl, and linking group, or any combinations thereof. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

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