4,7-dichlorofluorescein dyes as molecular probes

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai

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060967234

ABSTRACT:
Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2',4',5', 7'-tetrachloro-4,7-dichloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.

REFERENCES:
patent: 4318846 (1982-03-01), Khanna et al.
patent: 4439356 (1984-03-01), Khanna et al.
patent: 4481136 (1984-11-01), Khanna et al.
patent: 4647578 (1987-03-01), Crounse et al.
patent: 4855225 (1989-08-01), Fung et al.
patent: 4933471 (1990-06-01), Lee
patent: 4997928 (1991-03-01), Hobbs, Jr.
patent: 5066580 (1991-11-01), Lee
patent: 5171534 (1992-12-01), Smith et al.
patent: 5188934 (1993-02-01), Menchen et al.
patent: 5654442 (1997-08-01), Menchen et al.
patent: 5885778 (1999-03-01), Menchen et al.
Connel et al., "Automated DNA Sequence Analysis," BioTechniques, 5(4): 342-348 (1987).
Karger et al., "Multiwavelength Fluorescence Detection for DNA Sequencing Using Capillary Electrophoresis," Nucleic Acids Research, 19(18): 4955-4962 (1991).
Wehry, chapter 3 in Guilbault, editor, Practical Fluorescence, 2nd. Ed., (Marcel Dekker, New York, 1990).
Wehry, chapter 4 in Guilbault, editor, Practical Fluorescence, 2nd. Ed., (Marcel Dekker, New York, 1990).
Haugland, chapter 2 in Steiner, editor, Excited States of Biopolymers (Plenum Press, New York, 1983).
Lee et al., "Vita Blue: A New 644-nm Excitable Fluorescent Dye for Cell Analysis," Cytometry, 10:151-164 (1989).
Pringle, et al., "Automated Nucleotide Sequence Analysis Accuracy Is Strongly Dependent Upon Optimal Template Levels and Avoidance of Gel Overload," DNA Core Facilities Newsletter, 1:15-21 (1988).
Kaambara, et al., "Optimization of Parameters in a DNA Sequenator Using Fluorescence Detection," Biotechnology, 6:816-821 (1988).
Smith, et al., "Fluorescence Detection in Automated DNA Sequence Analysis," Nature, 321:674-679 (1986).
Prober, et al., "A System for Rapid DNA Sequencing with Fluorescent Chain-Terminating Dideoxynucleotides," Science, 238:336-341 (1987).
Kirkbright, G.F., "Chapter 9. Fluorescent Indicators," from Indicators (Bishop, E., ed., Pergamon Press, Oxford et al., pp. 685-698), 1987.
Marshall, P.N., "Rules for the Visible Absorption Spectra of Halogenated Fluorescein Dyes," Histochemical Journal, 7:299-303 (1975).
Smith et al, "The Synthesis and Use of Fluorescent Oligonucleotides in DNA Sequence Analysis", Methods in Enzymology, 155: 260-301 (1987).

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