3' protected nucleotides for enzyme catalyzed template-independe

Details 3' protected nucleotides for enzyme catalyzed template-independe 3' protected nucleotides for enzyme catalyzed template-independe

1995-06-07

1999-02-16

Wilson, James O.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 266, 536 267, C07H 1900

Patent

active

058722440

ABSTRACT:
A method for the stepwise creation of phosphodiester bonds between desired nucleosides resulting in the synthesis of polynucleotides having a predetermined nucleotide sequence by preparing an initiation substrate containing a free and unmodified 3'-hydroxyl group; attaching a mononucleotide selected according to the order of the predetermined nucleotide sequence to the 3'-hydroxyl of the initiating substrate in a solution containing a catalytic amount of an enzyme capable of catalyzing the 5' to 3' phosphodiester linkage of the 5'-phosphate of the mononucleotide to the 3'-hydroxyl of the initiating substrate, wherein the mononucleotide contains a protected 3'-hydroxyl group, whereby the protected mononucleotide is covalently linked to the initiating substrate and further additions are hindered by the 3'-hydroxyl protecting group. Methods in which a mononucleotide immobilized on a solid support is added to a free polynucleotide chain are also disclosed.

REFERENCES:
patent: 4096324 (1978-06-01), Kelly et al.
patent: 4423212 (1983-12-01), Skulnick
patent: 4605735 (1986-08-01), Miyoshi et al.
patent: 4689405 (1987-08-01), Frank et al.
patent: 4719176 (1988-01-01), Klotz
patent: 4816571 (1989-03-01), Andrus et al.
patent: 4820812 (1989-04-01), Miyoshi et al.
patent: 4863849 (1989-09-01), Melamede
patent: 4876335 (1989-10-01), Yamane et al.
patent: 4948882 (1990-08-01), Ruth
patent: 4950745 (1990-08-01), Ishido et al.
patent: 4980460 (1990-12-01), Molko et al.
patent: 5003059 (1991-03-01), Brennan
patent: 5032680 (1991-07-01), Kawai et al.
patent: 5039796 (1991-08-01), Engels et al.
patent: 5047524 (1991-09-01), Andrus et al.
patent: 5091519 (1992-02-01), Gruickshank
patent: 5093232 (1992-03-01), Urdea et al.
patent: 5112963 (1992-05-01), Pieles et al.
patent: 5256549 (1993-10-01), Urdea et al.
patent: 5258506 (1993-11-01), Urdea et al.
patent: 5262530 (1993-11-01), Andrus et al.
patent: 5262536 (1993-11-01), Hobbs, Jr.
patent: 5264563 (1993-11-01), Huse
patent: 5264566 (1993-11-01), Froehler et al.
patent: 5268266 (1993-12-01), Fritsch et al.
patent: 5268464 (1993-12-01), Brill
patent: 5281701 (1994-01-01), Vinayak
patent: 5348868 (1994-09-01), Reddy et al.
patent: 5362866 (1994-11-01), Arnold
patent: 5367066 (1994-11-01), Urdea et al.
patent: 5380833 (1995-01-01), Urdea
patent: 5436143 (1995-07-01), Hyman
patent: 5652099 (1997-07-01), Conrad
Hyman, Edward David, The Hyman Method: Oligonucleotide Synthesis and Plasmid Preparation, 1995.
Bollum, Fed. Proc. Soc. Exp. Biol. Med., 17, 193 (1958).
Deng and Wu, Meth. Enzymol., 100: 96-116 (1983).
Kaufmann et al., Eur. J. Biochem, 24:4-11 (1971).
Hinton and Gumport, Nucleic Acid Res., 7:453-464 (1979).
Modak, Biochemistry, 17, 3116-3120 (1978).
England and Uhlenbeck, Biochemistry, vol. 17, 11:2069-2076 (1978).
Chang and Bollum, Biochemistry, vol. 10, 3:536-542 (1971).
Bennett et al., Biochemistry, vol. 12, 20:3956-3960 (1973).
Kossel and Roychoudrury, Eur. J. Biochem, 22:271-276 (1971).
Flugel et al., Biochem. Biophys. Acta., 308:35-40 (1973).
Sarfati et al., J. Biol. Chem., 265(31), 18902-18906 (1990).
Metzker et al., Nucleic Acids Res., 22(20), 4259-4267 (1994).
Canard et al., Proc. Nat'l. Acad. Sci. USA, 92, 10859-10863 (Nov. 1995).

Affiliated with

Also associated with

Rating

3' protected nucleotides for enzyme catalyzed template-independe does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with 3' protected nucleotides for enzyme catalyzed template-independe, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and 3' protected nucleotides for enzyme catalyzed template-independe will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2063463