3-methoxy-4-hydroxyphenylglycol fluorescence polarization immuno

Details 3-methoxy-4-hydroxyphenylglycol fluorescence polarization immuno 3-methoxy-4-hydroxyphenylglycol fluorescence polarization immuno

1991-09-30

1992-09-08

Kepplinger, Esther L.

Chemistry: analytical and immunological testing

Involving producing or treating antigen or hapten

Producing labeled antigens

435 71, 436447, 436547, 436548, 530363, 5303898, 5303892, 549223, 549225, G01N 33533, A61K 39385, A61K 39395

Patent

active

051457911

ABSTRACT:
The present invention is directed to a fluoroescence polarization immunoassay for determining the 3-methoxy-4-hydroxyphenylglycol content in body fluids, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for preparing them. The assay is conducted by measuring the degree of polarization of plane polarized light that has been passed through a sample containing antiserum and tracer.

REFERENCES:
patent: 4420568 (1983-12-01), Wang
patent: 4476229 (1984-10-01), Fino
patent: 4510251 (1985-04-01), Kirkemo
patent: 4585862 (1986-04-01), Wang
Keeton: Science 211, pp. 586-588, (1981).

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