3-hydroxy-3-methyl-glutaryl-coa synthase preparation with improv

Details 3-hydroxy-3-methyl-glutaryl-coa synthase preparation with improv 3-hydroxy-3-methyl-glutaryl-coa synthase preparation with improv

1994-09-13

1997-09-16

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

435 4, 435 691, 43525233, 4353201, 435 14, 435 29, 435 73, C12N 988, C12N 1560, C12N 1570, C12Q 1527

Patent

active

056680015

ABSTRACT:
A preparation of recombinant 3-hydroxy-3-methylglutaryl-CoA synthase is disclosed, wherein the preparation has at least 0.024 unitsg specific activity. Preferably, the preparation has at least 0.24 units/mg specific activity and is a crude cell extract. Preferably, at least 90% of the synthase molecules have not been substantially proteolytically cleaved. A preparation of recombinant HMG-CoA synthase wherein the preparation retains 50% activity after storage at 4.degree. C. for six months is also disclosed. A method of evaluating the efficacy of candidate anti-isoprene and anti-cholesterol drugs is also disclosed which comprises exposing the candidate drug to a recombinant 3-hydroxy-3-methylglutaryl CoA synthase preparation.

REFERENCES:
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