3.alpha.-hydroxysteroid oxidase and quantitative analysis of 3.a

Details 3.alpha.-hydroxysteroid oxidase and quantitative analysis of 3.a 3.alpha.-hydroxysteroid oxidase and quantitative analysis of 3.a

1987-06-19

1989-12-26

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 28, 435192, C12Q 126, C12Q 128, C12N 908

Patent

active

048898015

ABSTRACT:
Disclosed herein is a 3.alpha.-hydroxysteroid oxidase which oxidizes a 3.alpha.-hydroxysteroid into a 3-oxosteroid and hydrogen peroxide. The oxidase may preferably be obtained from a culture broth of Pseudomonas testosteroni (ATCC 11996). The oxidase is useful for the quantitative analysis of a 3.alpha.-hydroxysteroid. Method and reagent useful for the quantitative analysis of a 3.alpha.-hydroxysteroid are also disclosed.

REFERENCES:
patent: 4680259 (1987-07-01), Cumbo et al.
patent: 4737457 (1988-04-01), Evans et al.
Sigma Price List, Feb. 1986, Biochemical and Organic Compounds for Research and Diagnostic Clinical Reagents, p. 625, Item H7127.

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