3,4-dihydroxy-2-butanone 4-phosphate synthase

Details 3,4-dihydroxy-2-butanone 4-phosphate synthase 3,4-dihydroxy-2-butanone 4-phosphate synthase

1998-06-09

2000-06-13

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435196, 43525233, 435419, 536 232, C12Q 168, C12N 916, C12N 120, C07H 2104

Patent

active

060748303

ABSTRACT:
Through functional complementation of an Escherichia coli auxotroph, 3,4-dihydroxy-2-butanone 4-phosphate synthase (DS), an indispensable enzyme of the riboflavin biosynthetic pathway of the rice blast fungus Magnaporthe grisea, has been cloned. This invention relates to the isolation of the nucleic acid fragment that encodes the fungal DS protein. In addition, the invention also relates to the construction of chimeric genes encoding all or a portion of the Magnaporthe grisea DS protein, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of Magnaporthe grisea DS in a transformed host cell. Finally, the invention also relates the use of the Magnaporthe grisea DS protein as a tool for identifying chemical agents that could be useful as fungicides, antibiotics, or herbicides.

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