3'(2')-amino- or thiol-modified, fluorescent dye-coupled nucleos

Details 3'(2')-amino- or thiol-modified, fluorescent dye-coupled nucleos 3'(2')-amino- or thiol-modified, fluorescent dye-coupled nucleos

1994-01-18

1997-10-21

Wilson, James O.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 221, 536 231, 536 251, 536 253, 536 2626, 536 263, 536 266, 536 268, 536 2721, 536 276, 536 278, 536 2781, 536 281, 536 285, 536 2853, 536 2854, C07H 1906, C07H 1916, C07H 2100

Patent

active

056797850

ABSTRACT:
3'-(2')-Amino- or thiol-modified, fluorescent dye-coupled nucleosides, nucleotides and oligonucleotides, and a process for the preparation and the use thereof
The OH group located in the 3' and/or 2' position of a nucleoside, nucleotide or oligonucleotide is derivatized to an amino or thiol group and subsequently a fluorescent dye is coupled thereto. The resulting 3'- and/or 2'-amino- and thiol-modified nucleosides, nucleotides and oligonucleotides can then be used for the synthesis of complementary strands in the presence of a template strand or of oligonucleotides and for the detection of genetic material. They have the advantage that the fluorescent label need no longer be attached to the 5' end of the oligonucleotide or to the nucleobase, and thus need not be introduced during the chemical synthesis as in labeling techniques hitherto known, while the known and conventional methods have the disadvantage that only a few polymerases can be employed for the synthesis, the acceptance of the triphosphates by the polymerases diminishes and, moreover, a large substrate excess is necessary.

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