Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1994-06-10
1997-02-25
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435212, 435219, 530413, C12N 964, C12N 950, C07K 122
Patent
active
056058268
ABSTRACT:
An apoptosis-associated protease having a relative mass of 24 kilodaltons and a defined amino acid composition is disclosed, together with a method for its purification from a cytoplasmic extract of mammalian cells treated with an apoptosis-inducing agent, such as tumor necrosis factor-.alpha. or UV irradiation, that comprises affinity chromatography with the serine protease inhibitor DK120 followed by heparin-sepharose chromatography. The protease has activity against the elastase-like substrate MAAPV and is capable of inducing apoptosis in isolated U937 cell target nuclei.
REFERENCES:
patent: 5298407 (1994-03-01), Anderson et al.
patent: 5340935 (1994-08-01), Anderson et al.
patent: 5360893 (1994-11-01), Owens et al.
Billings, P. C., et al., 1988, Cancer Research 48: 1789-1802.
Wright, S. C., et al., 1993, Journal of Cellular Biochemistry, 53:222-233.
Larrick, J. W., et al., 1990, The FASEB Journal 4:3215-3223.
Yuan, J., et al., 1993, Cell, 75: 641-652.
Stewart, B. W., 1994, Journal of the National Cancer Institute, 86(17): 1286-1296.
Gold, R., et al., 1994, Laboratory Investigation, 71(2):219-225.
Schlegel, J., et al., 1995, FEBS Letters, 364: 139-142.
Zhivotovsky, B., et al., 1994, FEBS Letters, 351: 150-154.
Kwo, P., et al., 1995, The American Journal of Physiology, 268(4Ph 1): G613-G621.
Darmon, A. J., et al., 1994, The Journal of Biological Chemistry 269(51): 32043-32046.
Wang, E., 1995, Cancer Research, 55: 2284-2292.
Heusel, J. W., 1994, Cell, 76(6):977-987.
Miura, M., et al., 1993, Cell, 75: 653-660.
Wright, S. C., et al., 1993, The FASEB Journal, 7(8):1045-1051.
Wright, S. C., et al., 1994, The FASEB Journal, 8(9):654-660.
Wright, S. C., et al., 1994, Journal of Experimental Medicine, 180: 2113-2123.
Sobur, G., et al., 1983, BioTechniques, 1:198-203.
Weaver, V. M., et al., 1993, Biochemistry and Cell Biology 71(9-10): 488-500.
Chundrasekaran, S., et al., 1985, Analytical Biochemistry 150(1): 141-144.
Shi, L., et al., 1992, Journal of Experimental Medicine, 175: 553-566.
Shi, L., et al., 1992, Journal of Experimental Medicine, 176: 1521-1529.
Sarin, A. et al. "Protease Inhibitors Selectively Block T Cell Receptor-triggered Programmed Cell Death in a Murine T Cell Hybridoma and Activated Peripheral T Cells," (1993) The Journal of Experimental Medicine vol. 178 (15) pp. 1693-1700.
Squier, M. et al. "Calpain Activation in Apoptosis," (1994) Journal of Cellular Physiology vol. 159 (2) pp. 229-237.
Green, et al., "Apoptosis and Cancer," PPO Updates, vol. 8, No. 1, Jan. 1994, pp. 1-14.
Wright, et al., "Apoptosis and DNA Fragmentation Precede TNF-Induced Cytolysis in U937 Cells," J. of Cellular Biochem., 48:344-355 (1992).
Gerschenson, et al., "Apoptosis: A Different Type of Cell Death," The FASEB Journal, vol. 6, Apr. 1992, pp. 2450-2455.
Schwartzman, et al., "Inernucleosomal Deoxyribonucleic Acid Cleavage Activity in Apoptotic Tymocytes: Detection and Endocrine Regulation," Endocrinology, vol. 128, No. 2, pp. 1190-1197 (1991).
Duvall, et al., "Death and the Cell," Immunology Today, vol. 7, No. 4, 1986, pp. 115-119.
Larrick James W.
Wright Susan C.
Moore William W.
Panorama Research, Inc.
Wax Robert A.
LandOfFree
24 kilodalton cytoplasmic protease activating DNA fragmentation does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with 24 kilodalton cytoplasmic protease activating DNA fragmentation , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and 24 kilodalton cytoplasmic protease activating DNA fragmentation will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1973597