23S rRNA nucleic acid probes to mycobacterium kansasii

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 536 2432, 536 2433, 935 78, C12Q 168, C12P 1934, C07H 2104

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056816987

ABSTRACT:
This invention discloses hybridization assay probes for Mycobacterium kansasii comprised of an oligonucleotide of about 18 nucleotides. These probes hybridize to a variable region of the 23S rRNA gene of Mycobacterium kansasii. The oligonucleotide probes are complementary to the rRNA variable region of the rRNA gene. Such probe specificity offers a rapid, non-subjective method of identification and quantitation of a bacterial colony for the presence of selected rRNA sequences capable of distinguishing all strains of Mycobacterium kansasii.

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