Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1995-04-20
1998-02-10
Houtteman, Scott W.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 6, 435 912, 514 44, 536 245, 536 221, 536 231, 536 243, 536 2431, 536 2433, 536 251, C12N 510, C12Q 168, A61K 4800, C07H 2104
Patent
active
057168245
ABSTRACT:
A compound having the formula: ##STR1## wherein, R1 represents 2'-O-R.sub.3 -thio-R.sub.3 and/or 2'-C-R.sub.3 -thio-R.sub.3, wherein R.sub.3 is independently a moiety selected from a group consisting of alkyl, alkenyl, alkynyl, aryl, alkylaryl, carbocyclic aryl, heterocyclic aryl, amide and ester, X represents a base or H; Y represents a phosphorus-containing group; and R2 represents H, DMT or a phosphorus-containing group.
REFERENCES:
patent: 4987071 (1991-01-01), Cech et al.
patent: 5264562 (1993-11-01), Matteucci
patent: 5334711 (1994-08-01), Sproat et al.
patent: 5434257 (1995-07-01), Matteucci et al.
patent: 5495009 (1996-02-01), Matteucci et al.
Cech, "Ribozymes and Their Medical Implications," JAMA 260:3030-3034 (1988).
Collins and Olive, "Reaction Conditions and Kinetics of Self-Cleavage of a Ribozyme Derived From Neurospora S RNA," Biochemistry 32:2795-2799 (1993).
Egholm et al., "PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules," Nature 365:566-568 (1993).
Guerrier-Takada et al., "The RNA Moiety of Ribonuclease P Is the Catalytic Subunit of the Enzyme," Cell 35:849-857 (1983).
Hampel et al., "Hairpin' Catalytic RNA Model: Evidence for Helices and Sequences Requirement for Substrate RNA," Nucleic Acids Research 18:299-304 (1990).
Hampel and Tritz, "RNA Catalytic Properties of the Minimum (-)sTRSV Sequence," Biochemistry 28:4929-4933 (1989).
Haseloff and Gerlach, "Simple RNA Enzymes with New and Highly Specific Endoribonuclease Activities," Nature 334:585-591 (1988).
Jeffries and Symons, "A Catalytic 13-mer Ribozyme," Nucleic Acids Research 17:1371-1377 (1989).
Jones et al., "Synthesis and Binding Properties of Pyrimidine Oligodeoxynucleoside Analogs Containing Neutral Phosphodiester Replacements: The Formacetal and 3'-Thioformacetal Internucleoside Linkages," J. Org. Chem. 58:2983-2991 (1993).
Kim and Cech, "Three-dimensional model of the active site of the self-splicing rRNA precursor of Tetrahymena," Proc. Natl. Acad. Sci. USA 84:8788 (1987).
Medina et al., "A Mild Method for the Conversion of Alcohols to Methylthiomethyl Ethers," Tetrahedron Lett. 29:3773-3776 (1988).
Perreault et al., "Mixed Deoxyribo- and Ribo-Oligonucleotides with Catalytic Activity," Nature 344:565-567 (1990).
Perrotta and Been, "Cleavage of Oligoribonucleotides by a Ribozyme Derived from the Hepatitis .delta. Virus RNA Sequence," Biochemistry 31:16-21 (1992).
Pieken et al., "Kinetic Characterization of Ribonuclease-Resistant 2'-Modified Hammerhead Ribozymes," Science 253:314-317 (1991).
Quaedflieg et al., "An Alternative Route to the Preparation of (3'.fwdarw.5') Methylene Acetal Linked Di- and Trinucleosides," Synthesis pp. 627-633 (Jun. 1993).
Saville and Collins, "A Site-Specific Self-Cleavage Reaction Performed by a Novel RNA In Neurospora Mitochondria," Cell 61:685-696 (1990).
Saville and Collins, "RNA-Mediated Ligation of Self-Cleavage Products of a Neurospora Mitochondrial Plasmid Transcript," Proc. Natl. Acad. Sci. USA 88:8826-8830 (1991).
Scaringe et al., "Chemical synthesis of biologically active oligoribonucleotides using .beta.-cyanoethyl protected ribonucleoside phosphoramidites," Nucl Acids Res. 18:5433-5441 (1990).
Stein and Cheng, "Antisense Oligonucleotides as Therapeutic Agents--Is the Bullet Really Magical?," Science 261:1004-1012 (1993).
Torrence et al., "Targeting RNA for degredation with a (2'-5'(oligoadenylate-antisense chimeria," Proc. Natl. Acad. Sci. USA 90:1300-1304 (1993).
Uhlenbeck, "A Small Catalytic Oligoribonucleotide," Nature 328:596-600 (1987).
Usman and Cedergren, "Exploiting the chemical synthesis of RNA," TIBS 17:334-339 (1992).
Usman et al., "Automated Chemical Synthesis of Long Oligoribonucleotides Using 2'-O-Silylated Ribonucleoside 3'-O-Phosphoramidites on a Controlled-Pore Glass Support: Synthesis of a 43-Nucleotide Sequence Similar to the 3'-Half Molecule of an Escherichia coli Formylmethoionine tRNA," J. Am. Chem. Soc. 109:7845-7854 (1987).
Usman et al., "Chemical modification of hamnmerhead ribozymes: activity and nuclease resistance," Nucleic Acids Symposium Series 31:163-164 (1994).
Heidenreich et al, "High activity and stability of hammerhead ribozymes containing 2'-modified pyrimidine nucleosides and phosphorothioates", J. Biol. Chem. 269(3)2131-2138, Jan. 1994.
Pudlo et al, "Deoxyoligonucleotides containing 2'5' acetal linkages: synthesis and hybridization properties", Tetrahedron Lett. 35(50):9315-9318, 1994.
Zavgorodny et al, "1-alkylthioalkylation of nucleoside hydroxyl functions and its synthetic applications: A new versatile method in nucleoside chemistry", Tetrahedron lett. 32(51):7593-7596, 1991.
Beigelman Leonid
Karpeisky Alex
Fredman Jeffrey
Houtteman Scott W.
Ribozyme Pharmaceuticals Inc.
LandOfFree
2'-O-alkylthioalkyl and 2-C-alkylthioalkyl-containing enzymatic does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with 2'-O-alkylthioalkyl and 2-C-alkylthioalkyl-containing enzymatic , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and 2'-O-alkylthioalkyl and 2-C-alkylthioalkyl-containing enzymatic will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2076480