2-acrylamine-2-methyl-1-propanesulfonic acid enhancement of alka

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 5, 435 6, 435 74, 435 75, 435 792, 435 793, 435 794, 435 795, 435 21, G01N 3353

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054439582

ABSTRACT:
A first solution containing Tris-HCl, MgCl.sub.2, a homopolymer of 2-acrylamide-2-methylpropanesulfonate (AMPS) and agarose is applied to a filter having alkaline phosphatase to be detected. A second solution containing Tris-HCl, MgCl.sub.2, homopolymer of AMPS, 5-bromo-4-chloroindoxyl phosphate, nitroblue tetrazolium and agarose is applied onto a film formed by the first solution.

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