17-.alpha.and 17-.beta.substituted acyl-3-carboxy-3,5-dienes and

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Cyclopentanohydrophenanthrene ring system doai

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514171, 552610, 552557, 552553, 552548, 552540, 552502, A61K 31375, C07J 900

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active

056417659

DESCRIPTION:

BRIEF SUMMARY
This application is a 371 of PCT/US93/11241 filed Nov. 18, 1993.


FIELD OF THE INVENTION

The present invention relates to certain novel 17.alpha. and 17.beta. substituted acyl-3-carboxy 3,5-diene steroidal compounds, pharmaceutical compositions containing these compounds, and methods for using these compounds to inhibit steroid 5-.alpha.-reductase. Also invented are novel intermediates and processes useful in preparing these compounds.


DESCRIPTION OF RELATED ART

The class of steroidal hormones known as androgens is responsible for the physical characteristics that differentiate males from females. Of the several organs that produce androgens, the testes produce these hormones in the greatest amounts. Centers in the brain exert primary control over the level of androgen production. Numerous physical manifestations and disease states result when ineffective control results in excessive androgen hormone production. For example, acne vulgaris, seborrhea, female hirsutism, male pattern baldness and prostate diseases such as benign prostatic hypertropy are correlated with elevated androgen levels. Additionally, the reduction of androgen levels has been shown to have a therapeutic effect on prostate cancer.
Testosterone is the principal androgen secreted by the testes and is the primary androgenic steroid in the plasma of males. It now is known that 5-.alpha.-reduced androgens are the active hormones in some tissues such as the prostate and sebaceous gland. Circulating testosterone thus serves as a prohormone for dihydrotestosterone (DHT), its 5-.alpha.-reduced analogne, in these tissues but not in others such as muscle and testes. Steroid 5-.alpha.-reductase is a Nicotinamide Adenine dinucleotide Phosphate(NADPH)dependent enzyme that converts testosterone to DHT. The importance of this enzyme in male development was dramatically underscored by the discovery of a genetic steroid 5-.alpha.-reductase deficiency in male pseudohermaphrodites. Imperato-McGinley, J., et al., (1979), J. Steroid Biochem. 11:637-648.
Recognition of the importance of elevated DHT levels in various disease states has stimulated many efforts to synthesize inhibitors of this enzyme. Among the most potent inhibitors identified to date are 3-carboxy-androsta-3,5-diene steroidal derivatives.
A number of 5-.alpha.-reductase inhibitors are known in the art. For example, describes the inhibition of human steroid 5.alpha. reductase (EC 1.3.1.30) by 3 androstene-3-carboxylic acids; Levy, et al. describes the mechanism of enzyme inhibitor interation in the inhibition or rat liver steroid 5.alpha. reductase by 3-androstene-3-carboxylic acids; describes the inhibition of steroid 5.alpha. reductase by unsaturated 3-carboxysteroids; Levy, et al, describes the interaction mechanism between rat prostatic steroid 5-alpha reductase and 3-carboxy-17.beta.-substituted steroids; describes the new steroid class of A ring aryl carboxylic acids; describes the effect of inhibitors of steroid 5.alpha. reductase in benign prostatic hyperplasia, male pattern baldness and acne; and describes steroidal 3-carboxylic acid derivatives as useful 5.alpha. reductase inhibitors. describes steroidal 3-carboxylic acid derivatives as useful 5-.alpha.-reductase inhibitors. Inc.) describes steroidal 3-carboxylic acid derivatives as useful 5.alpha.-reductase inhibitors.
However, none of the above references specifically suggest that any of the novel steroidal 17.alpha. or 17.beta.-substituted acyl-3-carboxy-androsta-3,5-diene compounds of the present invention would have utility as potent testosterone 5-.alpha.-reductase inhibitors.


SUMMARY OF THE INVENTION

This invention relates to a compound of the formula I: ##STR1## wherein Z is .alpha. or .beta. ##STR2## in which A is a linear or branched, saturated or unsaturated hydrocarbon chain containing from 1-12 carbon atoms: and hydrocarbon chain containing from 1-12 carbon atoms substituted with one or more substituents selected from the group consisting of: aryloxy, alkoxy, acyloxy, amino, N-acylamino, nitro, cyano, oxo, haloge

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