16S rRNA oligonucleotide probes for the identification of sulfat

Details 16S rRNA oligonucleotide probes for the identification of sulfat 16S rRNA oligonucleotide probes for the identification of sulfat

1989-04-17

1991-09-17

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 27, 935 77, 935 78, C12Q 168, C07H 1512, C12N 1500

Patent

active

050494899

ABSTRACT:
Oligonucleotide probes complementary to the 16S rRNA molecule, suitably labelled, that bind to a characteristic portion of the 16S rRNA molecule of spore-forming sulfate reducing bacterial (SRB) and/or the non-spore forming SRB, Desulfovibrio desulfuricans, are described. The use of these probes in conjunction with "negative control probes" to detect these bacteria in the oilfield as well as any other environment in qualitative and quantitative assays is described. Use of "Universal C" primer as a probe to detect all bacteria for total bacterial assays in environmental samples is described.

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