11-Arachidonate-lipoxygenase mutants

Details 11-Arachidonate-lipoxygenase mutants 11-Arachidonate-lipoxygenase mutants

2007-02-20

2007-02-20

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S440000, C435S252300, C435S320100, C435S069100, C435S071100, C536S023200, C536S023600

Reexamination Certificate

active

10030464

ABSTRACT:
A method for producing a plant lipoxygenase (LOX) with modified positional specificity toward arachidonic acid and its use for hydroperoxylation of arachidonic acid. The LOX makes it possible to produce for the first time (11S,14Z,12E,8Z,5Z)-11-hydroperoxy-14,12,8,5-eicosatetraenic acids on a large scale. To this end, arachidonic acid is incubated as substrate with the LOX under appropriate conditions. Hydroperoxylation of the arachidonic acid is then effected, preferably at position 11, with secondary products which are hydroperoxylated at position 8, at position 5, or at positions 11 and 8 and 5.

REFERENCES:
patent: WO 00/60093 (2000-10-01), None
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Sequence Alignment.
ExPASy: lipoxygenase
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