1.alpha. (or 24R), 25-dihydroxy vitamin D.sub.3 derivatives

Details 1.alpha. (or 24R), 25-dihydroxy vitamin D.sub.3 derivatives 1.alpha. (or 24R), 25-dihydroxy vitamin D.sub.3 derivatives

1989-10-05

1992-05-26

Brown, Johnnie R.

Organic compounds -- part of the class 532-570 series

Organic compounds

9,10-seco-cyclopentanohydrophenanthrene ring system or...

564394, 436504, 436111, C07J 4100

Patent

active

051170183

ABSTRACT:
A novel 1.alpha. (or 24R), 25-dihydroxy vitamin D.sub.3 amino acid derivative of the formula ##STR1## wherein R.sub.1 is OH or --O--CO--A--NH.sub.2, and R.sub.2 and R.sub.3 are each selected from the group consisting of hydrogen, OH and --O--CO--A--NH.sub.2 ; wherein one of R.sub.2 and R.sub.3 is hydrogen, one of R.sub.1, R.sub.2 and R.sub.3 is OH, and one of R.sub.1, R.sub.2 and R.sub.3 is --O--CO--A--NH.sub.2 ; and wherein A is C.sub.1-10 alkylene, is produced by removing a protective group for the amino group, e.g. 9-fluorenylmethyloxycarbonyl, in the presence of a base in an inert solvent. A radioisotope iodine-labeled residue is then attached to the amino group to produce a derivative useful in the assay of 1.alpha. (or 24R), 25-dihydroxy vitamin D.sub.3 in a specimen.

REFERENCES:
Yamamoto, et al "Monoclonal Antibody for Calcitriol (1.alpha.,25-Dihydroxyvitamin D.sub.3)" J. Biochem. 98 (1985) pp. 991-998.
"Synthesis of a photoaffinity-labeled analog of 1,25-dihydroxyvitamin D.sub.3 ", Chemical Abstracts, vol. 103, No. 19, Nov. 1985, By R. Ray et al., p. 685.
"Evaluation of a photolabile derivative of 1,25-dihydroxyvitamin D.sub.3 as a photoaffinity probe for 1,25-dihydroxyvitamin-D.sub.3 receptor in chick intestinal cytosol", Chemial Abstracts, vol. 104, No. 3, Jan. 1986, By R. Ray et al., p. 275.
"Chemical synthesis and biological evaluation of photoaffinity-labeled derivatives of vitamin D.sub.3, 25-hydroxyvitamin D.sub.3, and 1,25-dihydroxyvitamin D.sub.3 ", Chemical Abstracts, vol. 104, No. 19, May, 1986, By R. Ray et al. p. 323.
"Photoaffinity labeling of the rat plasma vitamin D binding protein with [26,27-.sup.3 H]-25-hydroxyvitamin D.sub.3 -3.beta.-[N-(4-azido-2-nitrophenyl) glycinate]", Chemical Abstracts, vol. 105, No. 11, Sep. 1986, By R. Ray et al., pp. 316-317.
"Photoactivable analogs for labeling 25-hydroxyvitamin D.sub.3 serum binding protein and for 1,25-dihydroxyvitamin D.sub.3 intestinal receptor protein", Chemical Abstracts, vol. 106, No. 9, Mar. 1987, By A. Kutner et al., p. 640.
"Synthesis and biologic evaluation of a second generation photoaffinity analog of 1,25-dihydroxyvitamin D.sub.3 ", Chemical Abstracts, vol. 111, No. 1, Jul. 1989, By R. Ray et al., p. 739.
"Multiple Assay of Vitamin D and It's Metabolites", Vitamins, vol. 55, No. 12, 1981, By. Y. Nishii et al., pp. 595-605.
Pharma Medica Approach, vol. 3, No. 1, 1985, pp. 89-96.
Hormone and Clinical Trails, vol. 33, No. 10, 1985, pp. 915-924.
"A Sensitive, Precise, and Convenient Method for Determination of 1,25-Dihydroxyvitamin D in Human Plasma", Archives of Biochemistry and Biophysics, vol. 176, 1976, By J. Eisman et al., pp. 235-243.
Clinical Chemistry, vol. 26, No. 1, 1982, pp. 7-18.
"A Radioimmunoassay for 1,25-Dihydroxycholecalciferol", Clinical Science and Molecular Medicine, vol. 54, 1978, By T. Clemens et al. pp. 329-332.
"A Modified Radioimmunoassay for 1,25-Dihydroxycholecalciferol", Clinical Chemistry, vol. 27, No. 3, 1981, By T. Gray et al., pp. 458-463.
"A Radioimmunoassay for 1,25-Dihydroxycholecalciferol", Clinical Chemistry, vol. 26, No. 5, 1980, By R. Bouillon et al., pp. 562-567.
"Radioimmunoassay for 1,25-Dihydroxycholecalciferol", Clinical Chemistry, vol. 29, No. 1, 1983, By T. Gray et al., pp. 196-200.
"Monoclonal Antibody with High Affinity for 1,25-Dihydroxycholecalciferol", Biochemical and Biophysical Research Communications, vol. 112, No. 2, 1983, By H. Perry III et al., pp. 431-436.

Affiliated with

Also associated with

Rating

1.alpha. (or 24R), 25-dihydroxy vitamin D.sub.3 derivatives does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with 1.alpha. (or 24R), 25-dihydroxy vitamin D.sub.3 derivatives, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and 1.alpha. (or 24R), 25-dihydroxy vitamin D.sub.3 derivatives will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-421429