Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Reexamination Certificate
2001-06-04
2003-10-07
Raymond, Richard L. (Department: 1624)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
C435S174000, C435S181000, C436S532000, C530S345000, C530S350000, C530S391700, C530S402000, C530S810000, C530S816000, C536S017100, C536S023100, C536S024300, C536S024500, C558S288000, C558S289000, C558S290000, C562S007000
Reexamination Certificate
active
06630577
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to the field of bioconjugate preparation, and more particularly, to a novel class of 1,2-phenylenediboronic acid (1,2-PDBA) reagents useful for the conjugation of biological macromolecules, and the method of making and using such reagents.
BACKGROUND OF THE INVENTION
Bioconjugation is a descriptive term for the joining of two or more different molecular species by chemical or biological means, in which at least one of the molecular species is a biological macromolecule. This includes, but is not limited to, conjugation of proteins, peptides, polysaccharides, hormones, nucleic acids, liposomes and cells, with each other or with any other molecular species that add useful properties, including, but not limited to, drugs, radionuclides, toxins, haptens, inhibitors, chromophores, fluorophores, ligands, etc. Immobilization of biological macromolecules is also considered a special case of bioconjugation in which the macromolecule is conjugated, either reversibly or irreversibly, to an insoluble support including a chromatographic support. Bioconjugation is utilized extensively in biochemical, immuno-chemical and molecular biological research. Major applications of bioconjugation include, but are not limited to, detection of gene probes, enzyme-linked immunological solid-phase assay, monoclonal antibody drug targeting and medical imaging.
Bioconjugates are generally classified as either direct or indirect conjugates. Direct conjugates encompass those in which two or more components are joined by direct covalent chemical linkages. Alternatively, indirect conjugates encompass those in which two or more components are joined via an intermediary complex involving a biological macromolecule.
Avidin-biotin System
Although numerous methods of indirect bioconjugate preparation have been described, a significant number of those reported in the literature have been prepared by exploiting the Avidin-Biotin system. In the Avidin-Biotin system, the binding specificity of the protein Avidin (purified from egg white), or Streptavidin (purified from the bacterium
Streptomyces avidinii
), toward the cofactor Biotin (vitamin H) is utilized to bridge an Avidin conjugated macromolecule with a biotinylated macromolecule. Both Avidin and Streptavidin possess four Biotin binding sites of very high affinity (K
d
=10
−15
mol
−1
).
The Avidin-Biotin system has been utilized extensively for enzyme-linked immuno-logical solid-phase assay (ELISA), in which an enzyme-Avidin conjugate (useful for detection by reaction with the enzyme's substrate to afford a colored or chemiluminescent product) is employed to detect the presence of a biotinylated antibody, after first binding the antibody to an immobilized antigen or hapten. Applications of the Avidin-Biotin system number in the hundreds, and have recently been reviewed (Wilchek, M. and Bayer, E. A., (1990)
Methods in Enzymology,
184).
Although utilized extensively, several limitations are known to be associated with the Avidin-Biotin system, which include nonspecific binding generally attributed to the basicity of the Avidin molecule, nonspecific binding attributed to the presence of carbohydrate residues on the Avidin molecule, and background interference associated with the presence of endogenous Biotin, which is ubiquitous in both eukaryotic and prokaryotic cells.
Digoxigenin Anti-digoxigenin System
An alternative indirect bioconjugation system designed to overcome some of the limitations associated with the Avidin-Biotin system has recently been developed for the detection of gene probes by ELISA. See Kessler, C., Hôltke, H.-J., Seibl, R., Burg, J. and Mühlegger, K.,
Biol. Chem. Hoppe
-
Seyler
(1990), 371, 917-965. This system involves the use of the steroid hapten Digoxigenin, an alkaloid occurring exclusively in Digitalis plants, and Fab fragments derived from polyclonal sheep antibodies against Digoxigenin (anti-Digoxigenin). The high specificity of the various anti-Digoxigenin antibodies affords low backgrounds and eliminates the non-specific binding observed in Avidin-Biotin systems. Digoxigenin-labeled DNA and RNA probes can detect single-copy sequences in human genomic Southern blots. The development of the Digoxigenin anti-Digoxigenin system has recently been reviewed. See Kessler, C. (1990) in Advances in Mutagenesis Research (Obe, G. ed.) pp. 105-152, Springer-Verlag, Berlin and Heidelberg. The Digoxigenin anti-Digoxigenin system is the most recent representative of several hapten-antibody systems now routinely utilized for bioconjugation.
Immobilized Phenylboronates
Phenylboronic acids are known to interact with a wide range of polar molecules having certain requisite functionalities. Complexes of varying stability, involving 1,2-diols, 1,3-diols, 1,2-hydroxy acids, 1,3-hydroxy acids, 1,2-hydroxylamines, 1,3-hydroxylamines, 1,2-diketones and 1,3-diketones, are known to form with either neutral phenylboronic acid or the phenylboronate anion. Consequently, immobilized phenylboronic acids have been exploited as chromatographic supports to selectively retain, from diverse biological samples, those molecular species having the requisite functionalities. Many important biological molecules including carbohydrates, catecholamines, prostaglandins, ribonucleosides, and steroids contain the requisite functionalities, and have been either analyzed or purified in this manner. The use of phenylboronic acid chromatographic media for the isolation and separation of biological molecules has been discussed in several reviews. See Singhal, R. P. and DeSilva, S. S. M.,
Adv. Chromatog
. (1989), 31, 293-335; Mazzeo, J. R. and Krull, I. S.,
BioChromatog
. (1989), 4, 124-130; and Bergold, A. and Scouten, W. H. (1983) in Solid Phase Biochemistry (Scouten, W. H. ed.) pp. 149-187, John Wiley & Sons, New York.
Phenylboronic acid, like boric acid, is a Lewis acid, and ionizes not by direct deprotonation, but by hydration to give the tetrahedral phenylboronate anion (pK
a
=8.86). Phenylboronic acid is three times as strong an acid as boric acid. Ionization of phenylboronic acid is an important factor in complex formation, in that, upon ionization, boron changes from trigonal coordination (having average bond angles of 120° and average bond lengths of 1.37 angstroms) to the tetrahedral coordination (having average bond angles of 109° and average bond lengths of 1.48 angstroms).
Molecular species having cis or coaxial 1,2-diol and 1,3-diol functionalities, and particularly carbohydrates, are known to complex with immobilized phenylboronate anion, to form cyclic esters under alkaline aqueous conditions. See Lorand, J. P. and Edwards, J. O.,
J. Org. Chem.
(1959), 24, 769.
Acidification of 1,2-diol and 1,3-diol complexes to neutral pH is know to release the diol containing species, presumably due to hydrolysis of the cyclic ester. Coplanar aromatic 1,3-diols, like 1,8-dihydroxynaphthalene, are known to complex even under acidic conditions due to the hydrolytic stability of six-membered cyclic boronic acid esters. See Sienkiewicz, P. A. and Roberts, D. C.,
J. Inorg. Nucl. Chem.
(1980), 42, 1559-1571.
Molecular species having pendant 1,2-hydroxylamine, 1,3-hydroxylamine, 1,2-hydroxy-amide, 1,3-hydroxyamide, 1,2-hydroxyoxime and 1,3-hydroxyoxime functionalities are also known to reversibly complex with phenylboronic acid under alkaline aqueous conditions similar to those associated with the retention of diol containing species. See Tanner, D. W. and Bruice, T. C.,
J. Amer. Chem. Soc.
(1967), 89, 6954.
General Methods for the Preparation of Phenylboronic Acids and Phenylenediboronic Acids
The most popular methods of synthesizing phenylboronic acids involve in situ generation of arylmagnesium or aryllithium species from aryl halides followed by transmetalation with a trialkoxyborate. See Todd, M. H., Balasubramanian, S. and Abell, C.,
Tetrahedron Lett
. (1997), 38, 6781-6784; Thompson, W. and Gaudino, J.,
J. Org. Chem
. (1984), 49, 5237-5243; Crisofoli, W. A. and Keay, B. A.,
Tetrahedron
Li Guisheng
Stolowitz Mark L.
Wiley Jean P.
Blakely , Sokoloff, Taylor & Zafman LLP
Prolinx, Inc.
Raymond Richard L.
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