1,2-dioxetane compounds as chemiluminescent labels for organic a

Details 1,2-dioxetane compounds as chemiluminescent labels for organic a 1,2-dioxetane compounds as chemiluminescent labels for organic a

1997-08-12

1999-02-09

Owens, Amelia

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

549332, 54830401, C07F 702, C07D30500

Patent

active

058696989

ABSTRACT:
Dioxetanes which couple with organic and biological molecules of the formula: ##STR1## wherein X is a leaving group which is removed by an `activating agent to produce light, wherein A is a coupling substituent, Ar is a substituent selected from the group consisting of phenyl and naphthyl to provide a label are described. R.sub.1 is an optional linker substituent and can have between 1 and 30 carbon atoms with some of the carbon atoms being oxygen, sulfur, nitrogen or phosphorus. Ar as phenyl is preferred. The dioxetane coupled molecules are useful in assays of all types where luminescence can be used as an indicator.

REFERENCES:
patent: 4857652 (1989-08-01), Schaap et al.
J.E. McMurry et al, J. Amer. Chem. Soc. 105, 1660 (1983).
J.E. McMurry, Chem. Rev., 89, 1513 (1989).
A.P. Schaap et al, Clin. Chem., 35, 1863 (1989).
H.R. Schroeder et al., Anal. Chem. 48, 1933 (1976).
H.R. Schroeder in Luminescent Immunoassays: Perspectives in Endocrinology and Clinical Chemistry, M. Serio and M. Pazzagli, Eds., Raven Press, New York, pp. 129-146 (1982).
M. Pazzagli et al., J. Steroid Biochem., 19, 407 (1983).
T.P. Whitehead et al., Nature, 305, 158 (1983).
G.H. Thorpe et al., Clin. Chem., 31, 1335 (1985).
I. Weeks et al., Clin. Chem., 29, 1474 (1983).
I. Weeks et al., Clin. Chem., 29, 1480 (1983).
L.J. Arnold, Jr., et al., Clin. Chem., 35, 1588 (1989).
J.C. Hummelen et al., Pure Appl. Chem., 59, 639 (1987).
J.C. Hummelen et al., "Thermochemiluminescent Immunoassays," in Complementary Immunoassays. W.P. Collins, Ed., Wiley & Sons, New York, p. 191 (1988).
J.C. Hummelen et al., Methods In Enzymology 133, 531 (1986).
H. Wynberg et al., "1,2-Dioxetanes as Chemilumescent Probes and Labels" in Bioluminescence and Chemiluminescence, M.A. DeLuca, W.D. McElroy, Eds., Academic Press, New York, p. 687 (1981).
L.J. Kricka, Ligand-Binder Assays, Marcel Dekker, Inc. New York, 1985, pp. 15-51.
M.Z. Atassi, "Chemical Modification and Cleavage of Proteins," Chap. 1 in Immunochemistry of Proteins, vol. 1, Plenum Press, New York, 1977, pp. 1-161.
L.J. Kricka, Ligand-Binder Assays, Marcel Dekker, Inc. New York 1985, pp. 18-20, Table 2.2.
T.H. Ji., Bifunctional Reagents, "Methods in Enzymology," 91, 580-609 (1983).
R.M. Buckland, Nature, 320, 557-558 (1986).
T.W. Greene, Protective Groups in Organic Synthesis, John Wiley, New York, 198, pp. 10-72 (1981).
A.P. Schaap et al., Tetrahedron Lett., 935 1987.
A.P. Schaap et al., Tetrahedron Lett, 1155 1987.
A.P. Schaap et al, Tetrahedron Lett, 1159 1987.

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