Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Patent
1985-08-15
1988-03-01
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
435174, 435177, 435180, 435182, C12P 1322, C12N 1102, C12N 1108, C12N 1104
Patent
active
047286113
ABSTRACT:
Phenylalanine is produced by contacting cells having transaminase activity with phenylpyruvic acid or phenylpyruvate in the presence of an amine donor. The cells may be ruptured or permeabilized to release their transaminase activity. Preferably, the cells are immobilized with a polyazetidine polymer. Preferred reaction conditions are an excess of amine donor in a ratio of at least 1.1:1 amine donor to phenylpyruvic acid or phenylpyruvate and a pH of 5-10 such as to convert at least 85% of the phenylpyruvic acid or phenylpyruvate to phenylalanine. Phenylalanine may also be produced from cinnamic acid using immobilized cells having phenylalamine ammonia lyase activity.
REFERENCES:
patent: 4304858 (1981-12-01), Wandrey et al.
patent: 4326031 (1982-04-01), Wandrey et al.
patent: 4436813 (1984-03-01), Wood et al.
patent: 4450233 (1984-05-01), Mimura et al.
patent: 4452892 (1984-06-01), Rosevear
patent: 4518692 (1985-05-01), Rozzell
Yamada et al., Applied and Environmental Microbiology, vol. 42, No. 5, 1981, pp. 773-778.
Jack et al., Advances in Biochem. Eng. vol. 5, 1977 pp. 126-135.
Oishi, K., The Microbial Production of Amino Acids, John Wiley & Sons, N.Y., 1972, pp. 435-452.
Porath, et al., Methods in Enzymology, vol. XLIV, 1976, pp. 19-45.
Calton Gary J.
Wood Louis L.
Naff David M.
Purification Engineering, Inc.
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