Method for monitoring T cell reactivity

Details Method for monitoring T cell reactivity Method for monitoring T cell reactivity

1996-05-31

1998-05-12

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 794, G01N 3353

Patent

active

057503567

ABSTRACT:
This invention provides a highly sensitive assay for the detection of T-cells reactive to an antigen by detecting a soluble factor whose secretion is induced by stimulation of the T-cell by the antigen. The assay includes an antigen-driven proliferation of specific T cells prior to restimulation with irradiated antigen presenting cells (APCs) and antigen. The assay can be performed on previously frozen PBMCs, providing greater convenience in sample processing, multiple use of a single sample as an internal standard, and simultaneous analysis of samples collected at different time points.

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