Production in Escherichia coli of extracellular Serratia spp. hy

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435199, 536 27, C12N 920, C12N 922, C12N 1555

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051734184

ABSTRACT:
Extracellular Serratia spp. enzymes have been found to be excreted by another gram-negative organism harbouring a plasmid carrying DNA from Serratia spp. encoding the enzymes. This organism, e.g. E. coli, is therefore employed to produce the enzymes, specific examples of Serratia spp. enzymes produced are a nuclease, a lipase and a phosopholipase. The nuclease may be employed to remove nucleic acids from a biological material.

REFERENCES:
patent: 3960659 (1976-06-01), Fazakerley
patent: 4039382, Thang et al.
patent: 4161424 (1979-07-01), Ando et al.
patent: 4495287 (1985-01-01), Uhlin et al.
patent: 4499189 (1985-02-01), Uhlin et al.
patent: 4614718 (1986-09-01), Seino et al.
Gunner S. Skogman et al. Factors Affecting the Biosynthesis of L-Tryptophan by Genetically Modified Strains of Escherichia coli, Journal of General Microbiology 130:3091-3100 (1984).
Chemical Abstracts 103: 19095b (USSR Pat. No. 1,146,321).
Chemical Abstracts 92: 176305s (Severina et al.).
Chemical Abstracts 96: 213050k (Roy).
Jacobs et al., Nature 313: 806-810, (1985).
Vasil et al. J. Bacteriol. 152: 431-440 (1982).
Pierre Cornelis et al., Cloning and Expression of a Bacillus coagulans Amylase Gene in Escherichia coli Mol. Gen. Genet, (1982) 186:507-511.
B. A. Cantwell et al., Molecular cloning and expression of a Bacillus subtilis.beta.-glucanase gene in Escherichia coli-Gene, 23:211-219 (1983).
Maria Yang et al., Nucleotide sequence of the amylase gene from Bacillus subtilis, Nucleic Acid Research, 11:237 (1983).
Legakes, et al., Chem. Abstract vol. 90, 18831q.
BRL Catalog, 1985, p. 25.
Isolation and Properties of an Exocellular Nuclease of Serratia marcescens Eaves et al., J. Bateriol., pp. 273-278, Jul. 20, 1962.
Chem. Abstracts, vol. 103 No. 66030.
"A Rapid Alkaline Extraction Method for the Osolation of Plasmid DNA" Methods in Enzymology, vol. 100, pp. 243-255.
Clegg et al. FEMS Microbiology Letters 1985, 27(3) pp. 257-262.
Emerick et al., Biotechnology, Feb. 84, V2, N2 pp. 165-168.
Journ. of Bacteriology, Jun. 1984, Dauenhauer et al., "Cloning and Expression in Escherichia coli of Serratia marseens . . . ", pp. 1128-1132.
Chemical abstract, vol. 99 (1983) abstract No. 18517 c. J. Biochem (Tokyo), 1983, 93, pp. 1287-1295 (Eng).
Maniatis, Molecular Cloning, 1982.
G. Bertani, J. Bact. 62, 1951, p. 293.
Clark, et al, J. Mol. Biol. 23, 1967, p. 99.
Maxam et al., Proc. Natl. Acad. Sci USA 74, 1977, pp. 560-564.
Sanger et al., Proc. Nat. Acad. Sci. USA 74, pp. 5463-5467.
J. Messing, Nucl. Acids, Res. 9, 1981, pp. 390-321.
Shine, Dalgarno, Nature 254, 1975, p. 34.
R. Simon, Biol. Technology, Nov. 1983, pp. 784-791.
U. Winkler, Molec. gen. Genet. 124, 1973, pp. 197-206.
J. E. L. Larsen, Gene 28, 1984, pp. 45-54.
Chang & Cohen, J. Bacteriol. 134, 1978, pp. 1141-1156.
E. Remaut et al., Gene 15, 1981, pp. 81-93.

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