Thermostable lipoprotein lipase, process for producing the same,

Details Thermostable lipoprotein lipase, process for producing the same, Thermostable lipoprotein lipase, process for producing the same,

1989-08-09

1992-12-22

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435183, 435 19, C12N 920, C12N 900, C12Q 144

Patent

active

051734176

ABSTRACT:
The present invention is directed toward a thermostable lipoprotein lipase capable of hydrolyzing triglycerides in lipoproteins to glycerol and fatty acids, a process for producing the thermostable lipoprotein lipase, and a blood triglyceride determining reagent containing the thermostable lipoprotein lipase. The thermostable lipoprotein lipase exhibits about 100% retention of the hydrolyzing activity when treated in a buffer having a pH of from about 4 to 7 at about 60.degree. C. for about 15 minutes and a glycerol forming activity/fatty acid forming activity ratio of at least about 15%. The process comprises cultivating a thermophilic actinomycetes, particularly Streptomyces 7825 (FERM P-9983, FERM BP-2489 and recovering lipoprotein lipase from the culture.

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