Immobilized antibody or antigen for immunoassay

Details Immobilized antibody or antigen for immunoassay Immobilized antibody or antigen for immunoassay

1980-03-13

1983-10-04

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 34, 435177, 435179, 435180, 436528, 436530, 436809, 436811, G01N 3354, C12Q 104, C12N 1102

Patent

active

044079435

ABSTRACT:
An antigen or antibody and an immunochemically neutral protein are immobilized in two stages on a microporous membrane coated with a protein such as zein or collagen. Immobilized antigen to Toxoplasma gondii is used in an immunoassay to detect the presence of antibody to Toxoplasma gondii in serum. Antibody that becomes bound to the immobilized antigen is detected with an antibody- enzyme conjugate. Immobilizing the immunochemically netural protein on the membrane in a second stage immobilization step minimizes nonspecific binding during the immunoassay.

REFERENCES:
patent: 3770380 (1973-11-01), Smith
patent: 3839153 (1974-10-01), Schuurs et al.
patent: 3951748 (1976-04-01), Devlin
patent: 4002532 (1977-01-01), Weltman et al.
patent: 4036946 (1977-07-01), Kleinerman
patent: 4039652 (1977-08-01), Adams et al.
patent: 4066512 (1978-01-01), Lai et al.
patent: 4111754 (1978-09-01), Park
patent: 4200690 (1980-04-01), Root et al.
Toussaint A. J., Improvement of the Soluble Fluoresant-Antibody Procedure, Experimental Parasitology, vol. 19, 1966, (pp. 71-76).
Toussaint et al. Soluble Antigen Fluorescent-Antibody Technique, Applied Microbiology, vol. 13, No. 4, 1965 (pp. 552-558).

Affiliated with

Also associated with

Rating

Immobilized antibody or antigen for immunoassay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Immobilized antibody or antigen for immunoassay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immobilized antibody or antigen for immunoassay will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-974291