Production of glyoxylic acid with glycolate oxidase and catalase

Details Production of glyoxylic acid with glycolate oxidase and catalase Production of glyoxylic acid with glycolate oxidase and catalase

1994-06-03

1995-08-08

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435190, 435192, C12P 740, C12N 904, C12N 908

Patent

active

054398134

ABSTRACT:
A process for the production of glyoxylic acid by reacting glycolic acid with oxygen in an aqueous solution in the presence of an amine buffer capable of forming a chemical adduct with glyoxylic acid, and glycolate oxidase and catalase immobilized or co-immobilized on an insoluble support is disclosed. The reaction is carried out at a pH of 7 to 10, preferably 8 to 9.5, an initial concentration of glycolic acid of 200 to 2500 mM, a concentration of amine wherein the initial mole ratio of amine to glycolic acid is within the range of 1.0 to 3.0, a concentration of immobilized catalase of 50 to 100,00 IU/mL, preferably 350 to 14,000 IU/mL, an oxygen pressure of up to 50 atmospheres, preferably 15 atmospheres, an immobilized glycolate oxidase concentration of about 0.01 to 10 IU/mL, preferably about 0.1 to 4 IU/mL, and a temperature of 0.degree. to 40.degree. C. preferably 5.degree. to 15.degree. C. Preferred insoluble immobilization supports are Eupergit C-250L and Eupergit C (Oxirane acrylic beads).

REFERENCES:
patent: 3281460 (1966-10-01), Gandon
patent: 4094928 (1978-06-01), Gaertner et al.
patent: 4146731 (1979-03-01), Ogahara et al.
patent: 4233452 (1980-11-01), Williams et al.
patent: 4235684 (1980-11-01), Harada et al.
patent: 4455371 (1984-06-01), Richardson et al.
patent: 4460686 (1984-07-01), Hartmeier
patent: 4670191 (1987-06-01), Kleiner et al.
patent: 4871669 (1989-10-01), Murray et al.
patent: 5126247 (1992-06-01), Palmer et al.
patent: 5219745 (1993-06-01), Anton et al.
patent: 5221621 (1993-06-01), Anton et al.
patent: 5851159 (1989-07-01), Fields et al.
Tolbert et al., "J. Biol. Chem." vol. 181 pp. 905-914 (1949).
Richarson et al., "J. Biol. Chem." vol. 236 pp. 1280-1284 (1961).
Clagette et al., "J. Biol. Chem.", vol. 178, pp. 977-987 (1961).
Zelitch et al., "J. Biol. Chem." vol. 201 pp. 707-718 (1953).
Robinson et al., "J. Biol. Chem.", vol. 237, pp. 2001-2009 (1962).
Frigerio et al., "J. Biol. Chem.," vol. 231 pp. 135-157 (1958).
Zelitch et al., "Methods in Enzymology" vol. 1 pp. 528-532 (1955).
Nishimura et al., "Arch. Biochem. Biophys." vol. 222 pp. 397-402 (1983).
Asker et al., "Biochim Biophys. Acta." vol. 761 pp. 103-108 (1983).
Emes et al., "Int. J. Biochem.", vol. 16 1373-1378 (1984).
Cederlund et al., "Eur. J. Biochem." vol. 172 pp. 523-530 (1988).
Lindquist et al., "J. Biol. Chem." vol. 264, pp. 3624-3628 (1989).
Yagai, "Methods of Biochemical Analysis," vol. 8 pp. 319-355 (1962).
Volokito et al., "J. Biol. Chem." vol. 262, 15825 (1987).
Macheroux et al., "Biochemistry", vol. 30 pp. 4612-4619 (1991).
Seah et al., "J. Biol. Chem." vol. 218 No. 8 p. 2880 (1973).
Pollack, et al., "J. Am. Chem. Soc.", vol. 102 pp. 6324-6336 (1980).
Kerr, M. N., Graves, D., "Phytochemistry," vol. 14 pp. 359-362 (1975).

Affiliated with

Also associated with

Rating

Production of glyoxylic acid with glycolate oxidase and catalase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Production of glyoxylic acid with glycolate oxidase and catalase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Production of glyoxylic acid with glycolate oxidase and catalase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-970614