Recombinant plasmid for the expression of L-phenylalanine ammoni

Details Recombinant plasmid for the expression of L-phenylalanine ammoni Recombinant plasmid for the expression of L-phenylalanine ammoni

1988-02-01

1990-08-07

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435320, 935 29, 935 73, C12N 121, C12N 1570

Patent

active

049467905

ABSTRACT:
A recombinant plasmid for the expression of phenylalanine ammonia-lyase (PAL) is constructed by incorporating therein a combined promoter comprising (a) the fusion promoter (the tac promoter) composed of the trp promoter minus 35 region and the lac UV-5 promoter minus 10 region and (b) the P.sub.L promoter of the lambda phage, the tac promoter and the P.sub.L promoter being connected so as to have the same directional property. This recombinant plasmid permits more efficient expression of PAL in Escherichia coli.

REFERENCES:
Gilbert, H. J.; Clark, I. N.; Gibson, R. K.; Stephenson, J. R.; and Tully, M., "Molecular Cloning of the Phenylalanine Ammonia-Lyase Gene from Rhodosporidium Toruloides in Escherichia Coli K-12", Journal of Bacteriology, Jan. 1985, pp. 314-320.
Anson, J. G.; Gilbert, H. J.; Oram, J. D.; and Minton, N. P., "Complete Nucleotide Sequence of the Rhodosporidium Toruloides Gene Coding for Phenylalanine Ammonia-Lyase", Gene 58(1987), 189-199.
Gene, vol. 58, No. 2-3, 1987, pp. 189-199, Anson et al., "Complete Nucleotide Sequence of the Rhodosporidium toruloides gene . . .".

Affiliated with

Also associated with

Rating

Recombinant plasmid for the expression of L-phenylalanine ammoni does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Recombinant plasmid for the expression of L-phenylalanine ammoni, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant plasmid for the expression of L-phenylalanine ammoni will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-960483