Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-11-12
2000-12-05
Ketter, James
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435183, 4352351, 435441, 435446, 530350, C12Q 168
Patent
active
061565091
ABSTRACT:
An improved method of mutanting DNA is provided comprising mutating a gene bearing phagemid in a host and infecting the host cell with a suitable helper phage to initiate packaging of the DNA into a phagemid. The packaged phagemid is then used to infect a second host cell. The method facilitates more efficient screening for genetic diversity. Also, the gene of interest is mutated and separable from the host cell which undergoes the mutational event reducing false positive results or other artifacts of general mutation techniques.
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International Search Report for PCT/US98/23278.
Genencor International Inc.
Glaister Debra J.
Ketter James
Stone Christopher L.
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