Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1986-08-13
1990-07-17
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 24, 435909, 436547, 436548, G01N 3353
Patent
active
049421263
ABSTRACT:
A means for identifying C. jejuni, from stool specimens, by the extraction and agglutination of its soluble antigen(s). More specifically, bacteria are concentrated from fresh (unpreserved) stool specimens, soluble antigen is released--preferably either by a specified heating protocol or by the action of a particular enzyme--and the antigen is detected by agglutination upon exposure to monoclonal, polyclonal or other corresponding antibodies. The method of the invention not only fosters efficiency in the laboratory but also provides rapid diagnosis of the presence of C. jejuni, so that appropriate patient treatment may begin at the earliest opportunity.
REFERENCES:
Biological Abstract 83 (11): 108788.
Davis et al., Microbiology, 3rd Edition (1980), "Vibrionaceal", pp. 665-668.
Stanier et al., Microbial World, (1976), "Campylobacter and Bdellovibrio", p. 609.
Wong et al., Journal of Clinical Microbiology, vol. 21, No. 5, pp. 702-707, May 1985.
McCarthy, L. in Rapid Detection and Identification of Infectious Agents, Kingsbury et al. (ed), pp. 165-175, 1985.
Garcia et al., Diagnostic Parasitology Clinical Laboratory Manual, pp. 12-18, 1979.
Buck et al., Journal of Clinical Microbiology, vol. 29, No. 6, pp. 1094-1098, Dec. 1984.
Martinez et al., Journal of Bacteriology, vol. 109, No. 3, pp. 1239-1246, Mar. 1972.
Lior et al., Journal of Clinical Microbiology, vol. 15, No. 5, pp. 761-768, May 1982.
Penner et al., Journal of Clinical Microbiology, vol. 12, No. 6, pp. 732-737, Dec. 1980.
Logan et al., Journal of Bacteriology, vol. 168, No. 2, pp. 739-745, Nov. 1986.
Wenman et al., Journal of Clinical Microbiology, vol. 21, No. 1, pp. 108-112, Jan. 1985.
Sigma Chemical Company Catalog, pp. 770-771, Feb. 1984.
Allegheny-Singer Research Institute
Kepplinger Esther M.
Spiegel Carol A.
LandOfFree
Method of extracting antigens of bacteria does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of extracting antigens of bacteria, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of extracting antigens of bacteria will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-94923