Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-10-29
2000-09-12
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
424 149, 424 153, 4241301, 4241411, 435 71, 435 75, 435 79, 536 221, 536 231, 536 243, 536 253, 935 78, 935 88, C12Q 168, A61K 39395, C07H 1900
Patent
active
061176311
ABSTRACT:
The present invention provides a method of detecting antigens, which comprises immobilizing an antigen to a solid support and contacting the solid support with a means for hybridizing a labeled dendrimer to the antibody, through an oligonucleotide complexed thereto. A directly oligonucleotide labeled primary antibody or an oligonucleotide labeled secondary antibody may be employed, and a conventionally labeled dendrimer can subsequently be hybridized to the oligonucleotide through one or more of the outer arms of the dendrimer. The present invention offers the advantage over conventional methods of antigen detection by providing multiple label molecules per antigen, thereby enhancing the observed signal associated with the label.
REFERENCES:
patent: 5175270 (1992-12-01), Nilsen et al.
patent: 5487973 (1996-01-01), Nilsen et al.
patent: 5635602 (1997-06-01), Cantor et al.
patent: 5656731 (1997-08-01), Urdea
Joerger et al, Analyte Detection with DNA-labeled antibodies and polymerase chain reaction, Clinical Chemistry, vol. 41, No. 9, pp. 1371-1377, Sep. 5, 1995.
Singh et al, Starburst dendrimers: enhanced performance and flexibility for immunoassays, Clinical Chemistry, vol. 40, No. 9, pp. 1845-1849, Sep. 1, 1994.
Housel James C.
Polyprobe, Inc.
Swartz Rodney P.
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