Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1988-12-30
1997-01-28
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435810, 436501, 536 221, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, C12Q 168
Patent
active
055976921
ABSTRACT:
A method for determining the presence and location in animal or plant tissue of a specific polynucleotide population comprising: (a) preparing a section of the tissue to be examined; (b) contacting the tissue section under hybridization conditions with a synthetic, labelled oligonucleotide probe which is complementary to a representative portion of the target polynucleotide; (c) removing unhybridized probe material from the tissue section; and (d) detecting or identifying the locations in the tissue section where labelling by hybridization of the labelled probe has occurred. A diagnostic kit for use in performance of the above method is also disclosed.
REFERENCES:
patent: 4401759 (1983-08-01), Rubin et al.
Sngerer et al. (1981) Nucleic Acids Res., vol. 9, No. 12, pp. 2819-2840.
Cox et al, (1984) Developmental Biology, vol. 101, pp. 485-502.
Hudson, P. et al., Endocrinology, 108 No. 1: 353-356 (1981).
Brahic, M., et al., Proc. Natl. Acad. Sci. 75: 6125-6129 (1978).
Brahic, M. et al. Cell, 26:123-128 (1981).
Szostak, J. W. et al., Methods in Enzymol. 68: 419-428 (1979).
Suggs, S. V., et al., Proc. Natl. Acad. Sci, 78:6613-6617 (1881).
Singer, R. H., et al, Proc. Natl. Acad. Sci, 79:7331-7335 (1982).
Bauman, J. J., et al. Jour. Histochem & Cytochem, 29:238-246 (1981).
Syostak et al., Specific Binding of a Synthetic Oligodeoxyribonuclestide to Yeast Cytochrome C on RNA, Nature vol. 265, Jan. 6, 1977, pp. 61-63.
Ruth et al, DNA, 3:173 (1984).
Chollet et al, Nucleic Acid Research, 13:1529 (1985).
Kempe et al, Nucleic Acids Research, 13:45 (1985).
Murasugi et al, DNA, 3:269 (1984).
McConaughy et al, Biochem. Biophys. ACTA, 149:180 (1967).
Tecott et al, "In Situ Hybridization: Application to Neurobiology", Oxford University Press, pp. 3-24 (1987).
Coghlan John P.
Niall Hugh D.
Penschow Jennifer D.
Tregear Geoffrey W.
Howard Florey Institute of Experimental Physiology and Medicine
Marschel Ardin H.
Zitomer Stephanie W.
LandOfFree
Hybridization histochemistry method for determining the presence does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Hybridization histochemistry method for determining the presence, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Hybridization histochemistry method for determining the presence will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-940745