Method of preparing a monoclonal antibody

Details Method of preparing a monoclonal antibody Method of preparing a monoclonal antibody

1997-12-05

2000-02-01

Ceperley, Mary E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

4351722, 436518, 436548, 5303881, 5303882, C12P 2104, C12N 1500, G01N 33543, G01N 3353

Patent

active

060201703

ABSTRACT:
The present invention relates to an efficient method of producing monoclonal antibodies against surface antigens of cells and viruses. The method accommodates for antigens which are present in only relatively small amounts, or antigens of which only very small amounts are available or antigens which easily lose their in vivo conformation. Thus the method according to the invention comprises a series of steps, comprising a step in which B-cells from a mammal injected with surface antigen-comprising material are enriched with respect to the relative number of specific B-cells and a step which comprises a small-scale fusion technique.

REFERENCES:
patent: 5162223 (1992-11-01), Brennan et al.
patent: 5223426 (1993-06-01), Skibbens et al.
Steenbakkers et al. J. Immunol. Methods 163: 33-40, 1993.
Steenbakkers et al. Hum. Antibod. Hybridoma 4: 166-173, 1993.
Vartdal et al. Transplantation 43: 366-371, 1987.
Kvalheim et al. Cancer Res. 47: 366-371, 1987.
Ossendorp et al. J. Immunol. Methods 120: 191-200, 1989.
Horton et al. J. Immunol. Methods 124: 225-230, 1989.
P. Steenbakkers et al., Journal of Immunological Methods, 152:1:69-77, 1992.
P. Steenbakkers et al., Molecular Biology Reports, 19:2:125-134, 1994.
M. Glasky et al., Hybridoma, 8:4:377-389, 1989.
R. Glaser et al., ACTA Biotechnologica, 12:4:259-268, 1992.

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