Methods for detecting nucleic acids by ligation of multiple olig

Details Methods for detecting nucleic acids by ligation of multiple olig Methods for detecting nucleic acids by ligation of multiple olig

1999-02-02

2000-02-01

Myers, Carla J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 915, 435 9152, 536 253, 536 2532, C12Q 168, C12P 1934, C07H 2104

Patent

active

06020138&

ABSTRACT:
Methods of synthesizing polynucleotides are disclosed involving the simultaneous ligation of a set of oligomer 5'-phosphates onto a template-bound primer. The set of these oligomers can be preselected to contain oligomers which are complementary to the template strand or the oligomers can be supplied as a library and allowed to self select. The synthesis by ligation can proceed unidirectionally or bidirectionally from the primer and can be used to synthesize both strands simultaneously by the use of two primers. The ligation is preferably performed with a ligase enzyme. The methods of synthesis are useful in a variety of applications, including cloning, amplification, labeling, diagnostic assays, mutation analysis and screening, gene expression monitoring and sequence analysis.

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