Method and instrument for the preparation of pollen for cryogeni

Plant husbandry – Process

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426419, 426465, 800200, A01G 700, A01H 500

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055968384

ABSTRACT:
The present invention relates to a method and instrument for the preparation of pollen and the development of a pollen bank for breeding purposes. More specifically, the invention relates to a method and instrument that permits pollen to be cryogenically stored in a viable state. Specifically, the present invention relates to a method of employing a heat or water or pressure related measurement to determine the readiness of the pollen for cryogenic storage.

REFERENCES:
patent: 4746071 (1988-05-01), Grunhoff et al.
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Morisset, C; Gazeau, C; Hansz, J; Dereuddre., J. "Importance of Actin Cytoskeleton Behavior During Preservation of Carrot Cell-Suspensions in Liquied-Nitrogen", pp. 35-47. Protoplasm, vol. 173, 1993.
Nirde, P; Delbos, M; Combes, D. "New Procedure for Storing Pollen at Low Temperatures", pp. 211-212. Plant Physiology and Biochemistry, 26 (2), 1988.
Barnabas, B; Kovacs, G.; Abranyi, A.; and Pfahler, P. "Effects of Pollen Storage by Drying and Deep-Freezing on the Expression of Different Agronomic Traits in Maize (Zea mays L.)", Kluwer Academic Publishers, Dordrecht--Printed in the Netherlands, pp. 221-225. Euphytica 39(3) 1988.
Collins, T. C.; Lertmongkol, V.; and Jones, J. P. "Pollen Storage of Certain Agronomic Species in Liquid Air", Crop Science, vol. 13, Jul.-Aug. 1973, pp. 493-494.
Barnabas, B Preservation of Maize Pollen Biotechnology in Agriculture and Forestry, vol. 25 Maize (ed. by Y. P. S. Bajaj) Springer-Verlag Berlin Heidelberg (1994).
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Barnabas, B. and Rajki, E. "Fertility of Deep-frozen Maize (Zea Mays L.) Pollen" Agriculture Research Institute of the Hungarian Academy of Sciences, Annals of Botany Company (1981).
Barnabas, B. and Rajki, E. "Storage of Maize (Zea Mays L.) Pollen at -196.degree.C in Liquid Nitrogen", Euphytica 25 (1976) pp. 747-752.
Nath, J. and Anderson, J. O. "Effect of Freezing and Freeze-Drying on the Viability and Storage of Lilium longiflorum L. and Zea mays L. Pollen", Cryobiology 12, pp. 81-88 (1975).
Daniel, L. "Retention of the Germinating Power of Pollen during Storage", Institute of Genetics of the Hungarian-Academy of Sciences, Budapest.
Connor, Kristina and Towill, Leigh. "Pollen-Handling Protocol and Hydration/Dehydration Characteristics of Pollen for Application to Long-Term Storage", Kluwer Academics Publisher 1993 pp. 77-84.
Walden, D. B. "Male Gametophyte of Zea mays L." Crop Science, vol. 7, Sep.-Oct. 1967 pp. 441-443.
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Barnabas et al (1984) Acta Bot. Hung 30 (3-4):329-332.

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