Process for sequencing nucleic acids

Details Process for sequencing nucleic acids Process for sequencing nucleic acids

1989-12-11

1992-06-23

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 436 94, 436501, 536 26, 536 27, 536 28, 536 29, 935 77, 935 78, C12Q 168, C12P 1934, G01N 3348, C07H 1512

Patent

active

051242478

ABSTRACT:
For the sequencing of nucleic acids by enzymatic extension of an oligonucleotide primer in the presence of a polymerase, the four deoxyribonucleoside triphosphates, one of the four dideoxynucleoside triphosphates and the nucleic acid to be sequenced as template in each of four different preparations, labelling of the nucleic acid fragments which form and which are dependent on the dideoxynucleoside triphosphate used, separation by gel electrophoresis of the preparations containing the fragments and detection of the sequence via the label, at least two of the dideoxynucleoside triphosphates are used in different amounts together in one preparation and the fragments are differentiated by the intensity of the labelling signal of the bands in the gel or in another separation procedure.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
Ansorge et al., Nuc. Acids Res. 15(11): 4593-4602 (Jun. 11, 1987).
Ansorge et al., Nuc. Acids Res. 18(11): 3419-3420 (1990).
Kristensen et al., Nuc. Acids Res. 16(8): 3487-3496 (1988).

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