Isolation of unreduced oligosaccharides

Details Isolation of unreduced oligosaccharides Isolation of unreduced oligosaccharides

1985-09-06

1988-01-12

Griffin, Ronald W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 187, 536123, C07H 1900, C07H 2100

Patent

active

047192941

ABSTRACT:
A preparative scale method for the isolation of unreduced oligosaccharides of glycoproteins and glycohormones having an N-linked oligosaccharide structure is disclosed. The method employs hydrazinolysis of the glycoprotein or glycohormone under reaction conditions to cause cleavage at the N-linked sites, producing a mixture having as a major component a de-N-acetylated hydrazone derivative of the oligosaccharides, followed by N-acylation of the hydrazone derivative, acid-catalysis of the hydrazone derivative to produce unreduced oligosaccharides, and subjecting the resulting unreduced oligosaccharides to cellulose column chromatography to remove contaminants and to recover the unreduced oligosaccharides.

REFERENCES:
patent: 4362720 (1982-12-01), Lemieux et al.
Takasaki et al., Meth. Enzymol. 83, 263-268 (1982).
Yamashita et al., Ibid.; 105-126 (1982).
Mellis et al., Anal. Biochem. 114, 276-280 (1980).
Mizuochi et al., J. Biol. Chem. 251, 7404-7409 (1978).
Homans et al., Biochim. Biophys. Acta, 760, 256-261 (1983).
Homans et al., Proc. Natl. Acad. Sci. USA, 81, 6286-6289 (1984).

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