Measurement of diaphorase activity and reagent therefor

Details Measurement of diaphorase activity and reagent therefor Measurement of diaphorase activity and reagent therefor

1990-11-08

1992-11-24

Brown, Johnnie R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 25, 435 26, 435189, 435291, 435810, 435 19, 422 61, 422 681, C12Q 100, C12N 900

Patent

active

051660490

ABSTRACT:
A method for measuring diaphorase activity comprising mixing a sample comprising diaphorase with nitro blue tetrazolium, EDTA or a salt thereof, at least one of nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate, and a surface active agent, to form an assay solution; and measuring an increase in absorbance, due to the formation of diformazan, in the assay solution and reagents used in a method for measuring diaphorase activity comprising a first solution, a second solution, EDTA, and a surface active agent, wherein the first solution comprises at least one of nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate and the second solution comprises nitro blue tetrazolium.

REFERENCES:
patent: 4622296 (1986-11-01), Yamanishi et al.
patent: 4889797 (1989-12-01), Amano et al.

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