Chemistry: analytical and immunological testing – Tracers or tags
Patent
1993-06-21
1994-12-27
Nucker, Christine M.
Chemistry: analytical and immunological testing
Tracers or tags
422 58, 422 59, 422 8207, 422 8208, 436514, 436515, 436529, 436530, 436535, G01N 2164, G01N 2100, C12Q 100
Patent
active
053765516
ABSTRACT:
Apparatus for studying the interaction of first and second molecules in a test solution containing fluorescently labeled molecules in addition to the first and second molecules, with the first molecules and the fluorescently labeled molecules being capable of binding with the second molecules. The apparatus comprises a flow channel having opposite, spaced apart walls, with at least one of the walls or a portion thereof being translucent or transparent. A porous matrix is retained in a fixed position between the walls of the flow channel and in direct contact with a translucent or transparent portion of the walls of the flow channel. A test solution flows through the flow channel and around the porous matrix so as to be in contact with the porous matrix. The porous matrix comprises a material that is translucent or transparent and has pores or openings therein which are of a microscopic size such that the unbound fluorescently labeled molecules can permeate the porous matrix, while the second molecules and fluorescently labeled molecules bound to the second molecules cannot permeate the porous matrix. The porous matrix is substantially inert and does not react chemically with any of the molecules contained in the solution. Fluorescence emitted by the fluorescently labeled molecules permeating the porous matrix is detected through the translucent or transparent portion of the walls of the flow channel.
REFERENCES:
patent: 4138474 (1979-02-01), Updike
patent: 4257884 (1981-03-01), Lim
patent: 4348894 (1983-08-01), Yamamoto
patent: 4752562 (1988-06-01), Sherman et al.
patent: 4844869 (1989-07-01), Glass
patent: 4874691 (1989-10-01), Chandler
patent: 5063178 (1991-11-01), Toomey
patent: 5143066 (1992-09-01), Komives et al.
patent: 5183740 (1993-02-01), Ligler et al.
patent: 5252494 (1993-10-01), Walt
Pharmacia Catalogue, 1988. pp. 86, 87 & 108. 1988.
Furr et al, Anal. Biochem. 171, 360-365. 1988.
Crellin Terry M.
Dubrule Chris
Nucker Christine M.
University of Utah Research Foundation
LandOfFree
Apparatus for using fluorescently labeled ligands in studying in does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Apparatus for using fluorescently labeled ligands in studying in, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Apparatus for using fluorescently labeled ligands in studying in will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-918832